From 2011.igem.org

General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Make It: Magnetosome Protocols

Gibson Cloning/Assembly

Gibson Purification

Electroporation (Transformation)

[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep) ]

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