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From 2011.igem.org

Contents 1 Bisphenol A 1.1 Bisphenol A and its effects on mammals 1.2 Bisphenol A degradation 1.3 Further applications of bisphenol A degrading BioBricks and enzymes 2 S-layer 3 NAD+-detection 4 References

Bisphenol A

Bisphenol A and its effects on mammals

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Bisphenol A degradation

There exist a lot of bacteria in nature that can degrade xenobiotic substances such as phenolic compounds or endocrine disruptors. In a lot of soil samples that were taken from contaminated soil to find organisms that do so, sphingomonads were extraordinarily often isolated ([http://www.springerlink.com/content/t717l15u85507706/ Stolz, 2009]). In 2005, [http://www.springerlink.com/content/q7864l02734wg32m/ Sasaki et al.] isolated a soil bacterium from the Sphingomonas genus which is able to degrade the endocrine disruptor bisphenol A (BPA) with a unique rate and efficiency compared to other BPA degrading organisms. This strain, called Sphingomonas bisphenolicum AO1, is able to completely decompose 120 mg BPA L^-1 in about 6 hours while other strains need days of cultivation (e.g. Sphingomonas strain BP-7 isolated by [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article Sakai et al. (2007)]).

Further applications of bisphenol A degrading BioBricks and enzymes

S-layer

NAD⁺-detection

References

Sakai K, Yamanaka H, Moriyoshi K, Ohmoto T, Ohe T (2007) Biodegradation of Bisphenol A and Related Compounds by Sphingomonas sp. Strain BP-7 Isolated from Seawater, Bioscience, Biotechnology, and Biochemistry [http://www.jstage.jst.go.jp/article/bbb/71/1/71_51/_article 71(1):51-57].

Sasaki M, Maki J, Oshiman K, Matsumura Y, Tsuchido T (2005a) Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1, [http://www.springerlink.com/content/q7864l02734wg32m/ Biodegradation 16(5):449-459].

Stolz A (2009) Molecular characteristics of xenobiotic-degrading sphingomonads, Appl Microbiol Biotechnol [http://www.springerlink.com/content/t717l15u85507706/ 81:793-811].