September, 5th
T9002-ENTERO and ENTERO-4C5 were diluted 1:200 and 1:800 to perform two different tests on supernatants (collected on on August, 31st and on on September, 3rd).
Samples of dry DNA were prepared for R0040 in J61002-1C3-1 and J101-31-1C3-1 and sent to BMR genomics for sequencing.
E31-1, E41N-1, E42-1, E43-2, E43-3 were washed in 1 ml of M9 + Cm12.5 in order to remove 3OC6-HSL due to PtetR leakage; then they were diluted 1:100 in 4 ml volume (falcon tube). After two hours they were induced with a final concentration of 100 ng/ml atc. Supernatants were collected at the moment of induction, named t = 0 h and then at t = 2 h, t = 4 h and t = 6 h.
September, 6th
September, 7th
September, 8th
September, 9th
September, 10th
September, 11th
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