Team:UNIPV-Pavia/Calendar/August/week1

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UNIPV TEAM 2011

March
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7 8 9 10 11 12 13
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April
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18 19 20 21 22 23 24
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May
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2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
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11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
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15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
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19 20 21 22 23 24 25
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October
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31

AUGUST: WEEK 1

August, 1st

For each plate from the previous day, two colonies were picked.

Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes.

Screening (E-S) bande 1-2-3.
Trasformati in MGZ1 da MiniPrep.

August, 2nd

MODIFICARE: piccate J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate)

Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:

Plasmid DNA (ng/μl)
E28AGAIN-1 21.4
E28AGAIN-2 21.0
E41AGAIN-1 33.7
E41AGAIN-2 27.7
E37-2 18.5
E38-1 26.0
E39-1 21.6
E40-2 19.9
E42-1 25.1