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Thursday, July 21
From 2011.igem.org
Tube Prep
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.
These tubes were from the Promoter Plate
- P1
- P2
- P3
- P4
These tubes were from the Terminator Plate
- T1
- T2
- T3
- T4
These tubes were from the Ribosome Binding Site Plate
- R1
- R2
- R3
- R4
Incubation
The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.
Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B
- 5.0 µl PCR reserved Product
- 12.5 µl 10x Buffer
- 107.5 µl Nuclease free water
- 125.0 µl Total
Hours
