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From 2011.igem.org

-Molecular Genetics Microbiology- Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell. Amplified and purification I20260 with PCR. Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI