"

From 2011.igem.org

Template:Https://2011.igem.org/User:Tabima

---

Protocols

PCR for 16s (10 µl)

Reactives	µl	µl
Buffer	2.5	1
MgCl2	2	0.8
DNTP´s	0.5	0.2
P1492R	0.5	0.2
P27F	0.5	0.2
Taq	0.25	0.1
DNA	2	0.8
H2O	17.75	6.8
Final Vol	26	10

PCR Pfx

Reactives	ul
H2O	17.2
Buffer PFX	3
MgSO4	0.6
dNTP´s	0.9
FW	0.9
RV	0.9
Enhancer	3
Taq PFX	0.5
ADN	3
Total Mix	30

Reactives

1X µl

3X µl

H2O

18.8

56.4

Buffer PFX

2.5

7.5

MgSO4

0.5

1.5

dNTP´s

0.75

2.25

FW (Pr3)

0.75

2.25

RV (Pr4)

0.75

2.25

PFX

0.2

0.6

ADN

0.75

2.25

Mix Volume

25

75