"

From 2011.igem.org

Template:Https://2011.igem.org/User:Tabima

---

Contents 1 Protocols 1.1 PCR for 16s (10 µl) 1.2 PCR Pfx 1.3 PCR CBP 1.4 PCR Sensor

Protocols

PCR for 16s (10 µl)

Reactives	µl	µl
Buffer	2.5	1
MgCl2	2	0.8
DNTP´s	0.5	0.2
P1492R	0.5	0.2
P27F	0.5	0.2
Taq	0.25	0.1
DNA	2	0.8
H2O	17.75	6.8
Final Vol	26	10

PCR Pfx

Reactives	ul
H2O	17.2
Buffer PFX	3
MgSO4	0.6
dNTP´s	0.9
FW	0.9
RV	0.9
Enhancer	3
Taq PFX	0.5
ADN	3
Total Mix	30

PCR CBP

Reactives	1X µl	3X µl
H2O	18.8	56.4
Buffer PFX	2.5	7.5
MgSO4	0.5	1.5
dNTP´s	0.75	2.25
FW (Pr3)	0.75	2.25
RV (Pr4)	0.75	2.25
PFX	0.2	0.6
ADN	0.75	2.25
Mix Volume	25	75

PCR Sensor

Reactives	µl
H2O	17.3
Buffer PFX	3
MgSO4	0.6
dNTP´s	0.9
FW (1)	0.9
RV (2)	0.9
PFX	0.4
Enhancer	3
ADN	3
Total Mix	30