Team:Colombia/Notebook/Protocols

From 2011.igem.org

iGem @ Colombia


Contents

Protocols

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10















PCR Pfx

Reactives ul
H2O 17.2
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW 0.9
RV 0.9
Enhancer 3
Taq PFX 0.5
ADN 3
Total Mix 30

















PCR CBP

Reactives 1X µl 3X µl
H2O 18.8 56.4
Buffer PFX 2.5 7.5
MgSO4 0.5 1.5
dNTP´s 0.75 2.25
FW (Pr3) 0.75 2.25
RV (Pr4) 0.75 2.25
PFX 0.2 0.6
ADN 0.75 2.25
Mix Volume 25 75
















PCR Sensor

Reactives µl
H2O 17.3
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW (1) 0.9
RV (2) 0.9
PFX 0.4
Enhancer 3
ADN 3
Total Mix 30