• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

Protocols

Molecular Biology

• Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
• Linear template- TetR.
• tetR mutation PCR: induce site-specific mutations on tetR.
• Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
• Competent Cells.
• Competent Cells. Protocol II (Inoue method).
• Transformation.
• Primers preparation.
• Gibson assembly.
• Gel purification.

Microfluidics

• PDMS two layer device fabrication
• MITOMI: Protein – DNA interactions
• Chemostat cell culture
• Klenow dsDNA synthesis