Team:UNICAMP-EMSE Brazil/Data

Data Page

In this section you can find a list of all the parts we made/used in the Stress Wars project. By clicking on each part link you will be redirected to the part page on the Registry of Standard Biological Parts. For more details about the experimental tests, go to our Results page.

How Our System Works

Data For Our Favorite New Parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554013 Main Page] - Protein Secretion system device, BBa_K554013: This device will be important to export the produced interleukins (IL-12 and IL-10) to the extracellular media. This is a new secretion system device, inspired on Stanford Team 2009. To read a complete description of why BBa_K554013 consists on a new Part, you can click HERE or in the [http://partsregistry.org/Part:BBa_K554013:Experience Experience section of this part]. We could successfuly prove that this part worked as expected through proving the secretion of a marker gene (GFP) under oxidative stress.

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554010 Main Page] SoxR device
2. [ Main Page]

Parts we have improved

1. [http://partsregistry.org/Part:BBa_K223062:Experience Experience - Part:BBa_K223062 (Stanford, iGEM 2009) We have created a SoxS linked secretion system inspired in the Stanford 2009 secretion system device and we proved that it worked.

Data For Pre-Existing Parts

The following parts were created but we have not tested them yet

We could not create an appropriate test for the following parts:

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554011 Main Page] - QseB and QseC device, BBa_K554011: QseC is the bacterial adrenergic receptor which contains an histidine sensor kinase domain and QseB is its response regulator partner. This device is composed by a constitutive promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], RBS-QseC-RBS-QseB ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554006 BBa_K554006]) and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011 and http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K554014 Main Page] - flhDC-IL12-HlyA device, BBa_K554014: This part contains flhDC promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554001 BBa_K554001]), our fusion construct for Il-12 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554005 BBa_K554005]) linked to HlyA secretion signal peptide ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K554002 BBa_K554002] and terminator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 BBa_B0011 and http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 BBa_B0012]).

Some parts are under construction

Tested Devices (with GFP)

We created some devices with replacement of Il-10 and Il-12 by GFP. The following picture represents the systems which we could successfully test, with the following device codes:

UNICAMP-EMSE Table of Parts

Parts

Here you can find the parts submitted to registry. Each part was extensivelly described, click on the links below and have fun!

<groupparts>iGEM011 UNICAMP-EMSE_Brazil</groupparts>