Single-Cell Model

The single-cell model of our system consists of three connected but easily separable modules: the sensor, the band detector and the filter. The sensor detects toxic substances from cigarette smoke. The band detector produces green fluorescent protein upon a detection of a certain range (band) of toxic substance concentration. After the toxic substance concentration crosses a certain threshold, the filter turns the system red (red fluorescent protein is expressed). The single-cell model helped us understand in detail whether and how our synthetic circuit works, especially to explore the characteristics of the GFP band and get a feeling of how it is related to concentration of our input substance.