Team:Tokyo Metropolitan/Notebook/S14

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-Visualization-

-Electrophoresis

Digested samples since one day ago


-Ligation

Promoter - suppressed expression system


-Transformation

Above samples


@colony PCR oriTr-GFP-oriTr

@electrophoresis pSB1C3

@preculturefrom master plate of colony PCR


-Targeting-

Preculture

P0440(2010)

Colony PCR

Colony PCR J23100-K317037-K283030.

Electrophoresis

Electrophoresis colony PCR J23100-K317037-K283030 and find band only No.32.

Preculture

J23100-K317037-K283030 No.32