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Team:Tokyo Metropolitan/Notebook/S14
From 2011.igem.org
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-Visualization-
-Electrophoresis
Digested samples since one day ago
-Ligation
Promoter - suppressed expression system
-Transformation
Above samples
@colony PCR oriTr-GFP-oriTr
@electrophoresis pSB1C3
@preculturefrom master plate of colony PCR
-Targeting-
Preculture
P0440（2010）
Colony PCR
Colony PCR J23100-K317037-K283030.
Electrophoresis
Electrophoresis colony PCR J23100-K317037-K283030 and find band only No.32.
Preculture
J23100-K317037-K283030 No.32
