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Freezer Management
For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.
| Biobrick name | Description | Store | Length | Strain | Vector | Quality Control | Creation Date |
| BBa_K081022 | Plambda-RBS30-luxR-T(B1006)-pLux | BioBricks 2011 | 969* | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | 29/06/2011 |
| BBa_C0060 | (aiiA) autoinducer inactivation enzyme | BioBricks 2011 | 789* | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | 29/06/2011 |
| BBa_B0015 | (TT) double terminator | 2009 | 129 | E. coli TOP10 | pSB1AK3 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_R0040 | (pTet) TetR repressible promoter | 2009 | 54 | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_C0061 | (luxI) autoinducer synthetase for AHL | BioBricks 2011 | 618* | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | 30/06/2011 |
| BBa_I13501 | (mRFP-TT) Screening plasmid intermediate | requested from the Registry | 843* | E. coli TOP10 | pSB1AK3 | Seq ok (MIT) | FREEZER Requested 2011 |
| BBa_B0030 | RBS30 (eff=0.6) | 2009 | 15 | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_B0031 | RBS31 (eff=0.07) derivative of BBa_0030 | 2009 | 14 | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_B0032 | RBS32 (eff=0.3) / also used as non-fluorescent control in TECAN measurements (RBS) | 2009 | 13 | E. coli TOP10 / E. coli MGZ1 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_B0034 | RBS34 (eff=1) | 2009 | 12 | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| BBa_I13521 | (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP | 2010 | 923* | E. coli TOP10 | pSB1A3 | Seq ok (MIT) | FREEZER 2010 Dist |
| BBa_C0261 | (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) | BioBricks 2011 | 661* | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | 07/07/2011 |
| BBa_I13507 | (RBS34-mRFP-TT) Screening plasmid intermediate | 2009 | 861* | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | FREEZER 2009 Dist |
| pSB4C5 | Low copy BioBrick standard vector - chloramphenicol antibiotic resistance | 2009 | 3221 | E. coli TOP10 | pSB4C5 | Seq ok (MIT) | FREEZER 2009 Dist |
| pSB3K3 | Low to medium copy BioBrick standard vector - kanamycin resistance | BioBricks 2011 | 2750 | E. coli TOP10 | pSB3K3 | Seq ok (MIT) | 30/06/2011 |
| BBa_K3000005 | GFP (Silver Standard Prefix) with terminator | 2010 | 854 | E. coli TOP10 | pSB1C3 | Seq ok (BMR Gemonics) | FREEZER 2010 Ligations |
| BBa_K112808 in pSB4C5 | Enterobacteria phage T4 Lysis Device - no promoter / also used as non-fluorescent control in TECAN measurements (ENTERO4C5) | 2009 | 1785 | E. coli TOP10 / E. coli MGZ1 | pSB4C5 | Seq ok (BMR Gemonics) | FREEZER 2009 Ligations |
| T9002 | BBa_T9002 | 2009 | 1945 | E. coli TOP10 | pSB1A2 | Seq ok (MIT) | 2009 Registry Distribution |
| ENTERO-RBS | BBa_K112808 in pSB4C5 cotransformed with BBa_B0032 / also used as non-fluorescent control in TECAN measurements | 2011 | 1785 - 13 | E. coli TOP10 | pSB1A2 - pSB4C5 | Seq ok | FREEZER 2011 Ligations |
| T9002-ENTERO | BBa_T9002 cotransformed with BBa_K112808 in pSB4C5 / used as measurement device in TECAN measurements | 2011 | 1945 - 1785 | E. coli TOP10 | pSB1A2 - pSB4C5 | Seq ok | FREEZER 2011 Ligations |
| Biobrick name | Description | Ligation | Length | Strain | Vector | Quality Control | Creation Date |
| E1-2 | aiiA-TT | C0060(E-S)+B0015(E-X) | 951* | E. coli TOP10 | pSB1AK3 | Length OK | 07/07/2011 |
| E2-2 | RBS30-LuxI | C0061(X-P)+B0030(S-P) | 664* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E3-1 | RBS31-LuxI | C0061(X-P)+B0031(S-P) | 663* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E4-2 | RBS32-LuxI | C0061(X-P)+B0032(S-P) | 662* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E5-2 | RBS30-mRFP-TT | I13501(X-P)+B0030(S-P) | 864* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E6-1 | RBS31-mRFP-TT | I13501(X-P)+B0031(S-P) | 863* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E7-2 | RBS32-mRFP-TT | I13501(X-P)+B0032(S-P) | 862* | E. coli TOP10 | pSB1A2 | Length OK | 07/07/2011 |
| E8-3 | Plambda-RBS30-luxR-T(B1006)-pLux | K081022(E-P)+4C5(E-P) | 969* | E. coli TOP10 | pSB4C5 | Length OK | 07/07/2011 |
| E9-2 | RBS30-aiiA-TT | E1(X-P)+B0030(S-P) | 972* | E. coli TOP10 | pSB1A2 | Seq ok (BMR Genomics) | 13/07/2011 |
| E10-1 | RBS31-aiiA-TT | E1(X-P)+B0031(S-P) | 971* | E. coli TOP10 | pSB1A2 | Seq ok (BMR Genomics) | 13/07/2011 |
| E11-1 | RBS32-aiiA-TT | E1(X-P)+B0032(S-P) | 970* | E. coli TOP10 | pSB1A2 | Seq ok (BMR Genomics) | 14/07/2011 |
| E12-1 | RBS34-aiiA-TT | E1(X-P)+ B0034(S-P) | 969* | E. coli TOP10 | pSB1A2 | Seq ok (BMR Genomics) | 14/07/2011 |
| E13-1 | pTet-RBS30-LuxI | E2(X-P)+R0040(S-P) | 726* | E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 13/07/2011 |
| E14 | pTet-RBS31-LuxI | E3(X-P)+R0040(S-P) | 725* | E. coli TOP10 | pSB1A2 | FAILED | |
| E15 | pTet-RBS32-LuxI | E4(X-P)+R0040(S-P) | 724* | E. coli TOP10 | pSB1A2 | FAILED | |
| E16-1 | pTet-RBS34-LuxI | C0261(X-P)+R0040(S-P) | 723* | E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 13/07/2011 |
| E17-2 | Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT | E5(X-P)+E8(S-P) | 1841* | E. coli TOP10/ E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 13/07/2011 |
| E18-2 | Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT | E6(X-P)+E8(S-P) | 1840* | E. coli TOP10/ E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 13/07/2011 |
| E19-2 | Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT | E7(X-P)+E8(S-P) | 1839* | E. coli TOP10/ E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 13/07/2011 |
| E20-2 | Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT | I13507(X-P)+E8(S-P) | 1838* | E. coli TOP10/ E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 13/07/2011 |
| E21-1 | pTet-RBS30-mRFP-TT | E5(X-P)+R0040(S-P) | 926* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 14/07/2011 |
| E22-2 | pTet-RBS31-mRFP-TT | E6(X-P)+R0040(S-P) | 925* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 13/07/2011 |
| E23-1 | pTet-RBS32-mRFP-TT | E7(X-P)+R0040(S-P) | 924* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 14/07/2011 |
| BBa_R0040 in BBa_J61002 | pTet in BBa_J61002 plasmid | BBa_R0040(S-P)+BBa_J23101(S-P) | 923* | E. coli TOP10/ E. coli MGZ1 | BBa_J61002 | Length OK | 15/07/2011 |
| E24-2 | pTet-RBS30-aiiA-TT | E9(X-P)+R0040(S-P) | 1034* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 21/07/2011 |
| E25-1 | pTet-RBS31-aiiA-TT | E10(X-P)+R0040(S-P) | 1033* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 21/07/2011 |
| E26-2 | pTet-RBS32-aiiA-TT | E11(X-P)+R0040(S-P) | 1032* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 21/07/2011 |
| E27-2 | pTet-RBS34-aiiA-TT | E12(X-P)+R0040(S-P) | 1031* | E. coli TOP10/ E. coli MGZ1 | pSB1A2 | Seq ok (BMR Genomics) | 21/07/2011 |
| E28-1 | pTet-RBS30-luxI in pSB4C5 | E13(E-P)+pSB4C5(E-P) | 726* | E. coli MGZ1 | pSB4C5 | Bad Seq (BMR Genomics) | 21/07/2011 |
| E29 | pTet-RBS31-luxI in pSB4C5 | E14(E-P)+pSB4C5(E-P) | 725* | E. coli MGZ1 | pSB4C5 | FAILED | |
| E30 | pTet-RBS32-luxI in pSB4C5 | E15(E-P)+pSB4C5(E-P) | 724* | E. coli MGZ1 | pSB4C5 | FAILED | |
| E31-1 | pTet-RBS34-luxI in pSB4C5 | E16(E-P)+pSB4C5(E-P) | 723* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 21/07/2011 |
| E32-2 | pTet-RBS30-mRFP-TT in pSB4C5 | E21(E-P)+pSB4C5(E-P) | 926* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 21/07/2011 |
| E33-2 | pTet-RBS31-mRFP-TT in pSB4C5 | E22(E-P)+pSB4C5(E-P) | 925* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 21/07/2011 |
| E34-1 | pTet-RBS32-mRFP-TT in pSB4C5 | E23(E-P)+pSB4C5(E-P) | 924* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 21/07/2011 |
| E35-2 | pTet-RBS34-mRFP-TT in pSB4C5 | I13521(E-P)+pSB4C5(E-P) | 923* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 21/07/2011 |
| E36 | pTet-J61002 in pSB4C5 | pTet-J61002(E-P)+pSB4C5(E-P) | 923* | E. coli TOP10 | pSB4C5 | Length ok | 25/07/2011 |
| E37-2 | pTet-RBS30-aiiA-TT in pSB4C5 | E24(E-P)+pSB4C5(E-P) | 1034* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 29/07/2011 |
| E38-1 | pTet-RBS31-aiiA-TT in pSB4C5 | E25(E-P)+pSB4C5(E-P) | 1033* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 29/07/2011 |
| E39-1 | pTet-RBS32-aiiA-TT in pSB4C5 | E26(E-P)+pSB4C5(E-P) | 1032* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 29/07/2011 |
| E40-2 | pTet-RBS34-aiiA-TT in pSB4C5 | E27(E-P)+pSB4C5(E-P) | 1031* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 29/07/2011 |
| E41N-1 | pTet-RBS31-luxI in pSB4C5 | E36(S-P)+E3(X-P) | 725* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 04/08/2011 |
| E42-1 | pTet-RBS32-luxI in pSB4C5 | E36(S-P)+ E4(X-P) | 724* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 29/07/2011 |
| E43-3 | pTet-RBS30-luxI in pSB4C5 | E36(S-P)+ E2(X-P) | 726* | E. coli MGZ1 | pSB4C5 | Seq ok (BMR Genomics) | 25/08/2011 |
| E44-1 | pTet-RBS34-mRFP-TT in pSB1C3 | BBa_R0040 in BBa_J61002 (E-P) + pSB1C3 (E-P) | 923* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 04/09/2011 |
| J101-E5 | J23101-RBS30-mRFP-TT in pSB4C5 | BBa_J23101(S-P)+ E5(S-P) | 905* | E. coli MGZ1 | pSB4C5 | Length OK | 02/08/2011 |
| J101-31 | J23101-RBS31-mRFP-TT in pSB4C5 | BBa_J23101(S-P)+ E6(S-P) | 904* | E. coli MGZ1 | pSB4C5 | Length OK | 02/08/2011 |
| J101-E7 | J23101-RBS32-mRFP-TT in pSB4C5 | BBa_J23101(S-P)+ E7(S-P) | 903* | E. coli MGZ1 | pSB4C5 | Length OK | 02/08/2011 |
| J101-4C5 | J23101-RBS34-mRFP-TT in pSB4C5 | BBa_J23101(E-P)+ pSB4C5(E-P) | 902* | E. coli MGZ1 | pSB4C5 | Length OK | 02/08/2011 |
| E3O-1C3-3 | RBS31-LuxI | E3-1(E-P)+ pSB1C3 (E-P) | 663* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 07/09/2011 |
| E4N-1C3-3 | RBS32-LuxI | E4-2(E-P)+ pSB1C3 (E-P) | 662* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 11/09/2011 |
| E5-1C3 | RBS30-mRFP-TT | E5-2(E-P)+ pSB1C3 (E-P) | 864* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| E6-1C3 | RBS31-mRFP-TT | E6-1(E-P)+ pSB1C3 (E-P) | 863* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| E7-1C3 | RBS32-mRFP-TT | E7-2(E-P)+ pSB1C3 (E-P) | 862* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| E9-1C3 | RBS30-aiiA-TT | E9-2(E-P)+ pSB1C3 (E-P) | 972* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| E10-1C3 | RBS31-aiiA-TT | E10-1(E-P)+ pSB1C3 (E-P) | 971* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| E11-1C3 | RBS32-aiiA-TT | E11-1(E-P)+ pSB1C3 (E-P) | 970* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| J101-E5-1C3 | J23101-RBS30-mRFP-TT | J101-E5 (E-P) + pSB1C3(E-P) | 905* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 29/08/2011 |
| J101-31-C3-1 | J23101-RBS31-mRFP-TT | J101-31 (E-P) + pSB1C3(E-P) | 904* | E. coli TOP10 | pSB1C3 | Seq ok (BMR Genomics) | 04/09/2011 |
| J101-E7-1C3-1 | J23101-RBS32-mRFP-TT | J101-E7 (E-P) + pSB1C3(E-P) | 903* | E. coli TOP10 | pSB1C3 | Seq OK (BMR Gemonics) | 29/08/2011 |
* The sequences of these parts may contain at least a 25 bp barcode.
