Team:UNIPV-Pavia/Freezer

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UNIPV TEAM 2011

Freezer Management


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618* E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/2011
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843* E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) / also used as non-fluorescent control in TECAN measurements (RBS) 2009 13 E. coli TOP10 / E. coli MGZ1 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923* E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661* E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/2011
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861* E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/2011
BBa_K3000005 GFP (Silver Standard Prefix) with terminator 2010 854 E. coli TOP10 pSB1C3 Seq ok (BMR Gemonics) FREEZER 2010 Ligations
BBa_K112808 in pSB4C5 Enterobacteria phage T4 Lysis Device - no promoter / also used as non-fluorescent control in TECAN measurements (ENTERO4C5) 2009 1785 E. coli TOP10 / E. coli MGZ1 pSB4C5 Seq ok (BMR Gemonics) FREEZER 2009 Ligations
T9002 BBa_T9002 2009 1945 E. coli TOP10 pSB1A2 Seq ok (MIT) 2009 Registry Distribution
ENTERO-RBS BBa_K112808 in pSB4C5 cotransformed with BBa_B0032 / also used as non-fluorescent control in TECAN measurements 2011 1785 - 13 E. coli TOP10 pSB1A2 - pSB4C5 Seq ok FREEZER 2011 Ligations
T9002-ENTERO BBa_T9002 cotransformed with BBa_K112808 in pSB4C5 / used as measurement device in TECAN measurements 2011 1945 - 1785 E. coli TOP10 pSB1A2 - pSB4C5 Seq ok FREEZER 2011 Ligations


Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 951* E. coli TOP10 pSB1AK3 Length OK 07/07/2011
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 664* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 663* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 662* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969* E. coli TOP10 pSB4C5 Length OK 07/07/2011
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 972* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 971* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 970* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E12-1 RBS34-aiiA-TT E1(X-P)+ B0034(S-P) 969* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 726* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 725* E. coli TOP10 pSB1A2 FAILED
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 724* E. coli TOP10 pSB1A2 FAILED
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 723* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1841* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 926* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924* E. coli TOP10/ E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
BBa_R0040 in BBa_J61002 pTet in BBa_J61002 plasmid BBa_R0040(S-P)+BBa_J23101(S-P) 923* E. coli TOP10/ E. coli MGZ1 BBa_J61002 Length OK 15/07/2011
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1034* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1033* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1032* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1031* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 726* E. coli MGZ1 pSB4C5 Bad Seq (BMR Genomics) 21/07/2011
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 725* E. coli MGZ1 pSB4C5 FAILED
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 724* E. coli MGZ1 pSB4C5 FAILED
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 723* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 926* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923* E. coli TOP10 pSB4C5 Length ok 25/07/2011
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1034* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1033* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1032* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1031* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E41N-1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 725* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 04/08/2011
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+ E4(X-P) 724* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E43-3 pTet-RBS30-luxI in pSB4C5 E36(S-P)+ E2(X-P) 726* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 25/08/2011
E44-1 pTet-RBS34-mRFP-TT in pSB1C3 BBa_R0040 in BBa_J61002 (E-P) + pSB1C3 (E-P) 923* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 04/09/2011
J101-E5 J23101-RBS30-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E5(S-P) 905* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-31 J23101-RBS31-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E6(S-P) 904* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-E7 J23101-RBS32-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E7(S-P) 903* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-4C5 J23101-RBS34-mRFP-TT in pSB4C5 BBa_J23101(E-P)+ pSB4C5(E-P) 902* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
E3O-1C3-3 RBS31-LuxI E3-1(E-P)+ pSB1C3 (E-P) 663* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 07/09/2011
E4N-1C3-3 RBS32-LuxI E4-2(E-P)+ pSB1C3 (E-P) 662* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 11/09/2011
E5-1C3 RBS30-mRFP-TT E5-2(E-P)+ pSB1C3 (E-P) 864* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E6-1C3 RBS31-mRFP-TT E6-1(E-P)+ pSB1C3 (E-P) 863* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E7-1C3 RBS32-mRFP-TT E7-2(E-P)+ pSB1C3 (E-P) 862* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E9-1C3 RBS30-aiiA-TT E9-2(E-P)+ pSB1C3 (E-P) 972* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E10-1C3 RBS31-aiiA-TT E10-1(E-P)+ pSB1C3 (E-P) 971* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E11-1C3 RBS32-aiiA-TT E11-1(E-P)+ pSB1C3 (E-P) 970* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-E5-1C3 J23101-RBS30-mRFP-TT J101-E5 (E-P) + pSB1C3(E-P) 905* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-31-C3-1 J23101-RBS31-mRFP-TT J101-31 (E-P) + pSB1C3(E-P) 904* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 04/09/2011
J101-E7-1C3-1 J23101-RBS32-mRFP-TT J101-E7 (E-P) + pSB1C3(E-P) 903* E. coli TOP10 pSB1C3 Seq OK (BMR Gemonics) 29/08/2011

* The sequences of these parts may contain at least a 25 bp barcode.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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