From 2011.igem.org

General Protocols

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Agarose Gel Electrophoresis

Glycerol Stocks

Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Make It: Magnetosome Protocols

Gibson Reaction

Gibson Purification

Gibson Vectors (pGB) protocols

Wiki Design Tools