| March |
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T |
W |
T |
F |
S |
S |
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1 |
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10 |
11 |
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| 21 |
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24 |
25 |
26 |
27 |
| 28 |
29 |
30 |
31 |
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| April |
| M |
T |
W |
T |
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S |
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3 |
| 4 |
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6 |
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| 11 |
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| 18 |
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21 |
22 |
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24 |
| 25 |
26 |
27 |
28 |
29 |
30 |
| May |
| M |
T |
W |
T |
F |
S |
S |
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1 |
| 2 |
3 |
4 |
5 |
6 |
7 |
8 |
| 9 |
10 |
11 |
12 |
13 |
14 |
15 |
| 16 |
17 |
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19 |
20 |
21 |
22 |
| 23 |
24 |
25 |
26 |
27 |
28 |
29 |
| 30 |
31 |
| June |
| M |
T |
W |
T |
F |
S |
S |
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1 |
2 |
3 |
4 |
5 |
| 6 |
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8 |
9 |
10 |
11 |
12 |
| 13 |
14 |
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17 |
18 |
19 |
| 20 |
21 |
22 |
23 |
24 |
25 |
26 |
| 27 |
28 |
29 |
30 |
| October |
| M |
T |
W |
T |
F |
S |
S |
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1 |
2 |
| 3 |
4 |
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9 |
| 10 |
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| 24 |
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30 |
| 31 |
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- 04-08-2011 preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
- 05-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
- 17-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
- 18-08-2011 PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
- 20-08-2011 PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
- 23-08-2011 the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
- 24-08-2011 Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
- 25-08-2011 Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
- 26-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
- 29-08-2011 preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
- 03-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
- 05-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
- 05-09-2011 test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd (0 h, 7 h, 24 h)
- 06-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th (0 h, 2h, 6 h)
- 07-09-2011 test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th (0 h, 5 h, 24 h)
- 08-09-2011 test on T9002 sensitivity
- 09-09-2011 test on AiiA HC in MGZ1 (E24, E25, E26, E27 induced with 100 ng/ml atc) SN collected on Septmeber, 8th (0 h, 1 h, 2 h, 4 h, 7 h)
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