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AUGUST: WEEK 4
August, 22nd
Streak of E17-2, E18-2, J101-31, J101-E5 and ENTERO-4C5 on LB agar + Cm12.5 plate to test Plux promoter with different RBSs.
August, 23rd
T9002-ENTERO and ENTERO-RBS were diluted 1:500 in a final volume of 1 ml of M9 + Cm12.5.
August, 24th
E43 ligation was transformed in 100 μl of MGZ1 competent cells.
August, 25th
Three colonies were picked from E43 plate.
Every plasmid was digested with EcoRI and PstI resctriction endonucleases. A medium-size and a small-size agarose gel were prepared and, after three hours digestion, gel-electrophoresis was carried out:
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Team:UNIPV-Pavia/Calendar/August/week4
From 2011.igem.org
(Difference between revisions)
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<a name="August.2C_25th"></a><h2> <span class="mw-headline">August, 25th</span></h2> | <a name="August.2C_25th"></a><h2> <span class="mw-headline">August, 25th</span></h2> | ||
+ | <p> | ||
+ | Three colonies were picked from E43 plate. | ||
+ | <br> | ||
+ | Plasmid purification was carried out: | ||
+ | </p> | ||
+ | <center> | ||
+ | <table class="data"> | ||
+ | <tr> | ||
+ | <td class="row"><b>Plasmid</b></td> | ||
+ | <td class="row"><b>DNA (ng/μl)</b></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E2-2</td> | ||
+ | <td class="row">87.7</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E3-1</td> | ||
+ | <td class="row">83.9</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td class="row">E4-2</td> | ||
+ | <td class="row">93.4</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td class="row">E5-2</td> | ||
+ | <td class="row">89.7</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td class="row">E6-1</td> | ||
+ | <td class="row">80.6</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E7-2</td> | ||
+ | <td class="row">128.8</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td class="row">E9-2</td> | ||
+ | <td class="row">88.1</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E10-1</td> | ||
+ | <td class="row">68.5</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E11-1</td> | ||
+ | <td class="row">87.7</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">E36</td> | ||
+ | <td class="row">51</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">J101-E5</td> | ||
+ | <td class="row">25.7</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">J101-31</td> | ||
+ | <td class="row">56.1</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td class="row">J101-E7</td> | ||
+ | <td class="row">15.9</td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | </center> | ||
+ | |||
+ | <p> | ||
+ | Every plasmid was digested with EcoRI and PstI resctriction endonucleases. A medium-size and a small-size agarose gel were prepared and, after three hours digestion, gel-electrophoresis was carried out: | ||
+ | </p> | ||
Revision as of 22:03, 30 August 2011