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Team:UNICAMP-EMSE Brazil/Notebook/24 August 2011
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'''Result:''' | '''Result:''' | ||
*Very low concentration! New digestion to do with all the remaining from the Miniprep… | *Very low concentration! New digestion to do with all the remaining from the Miniprep… | ||
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| + | <img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | ||
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| + | function makeQuote() { | ||
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| + | Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)" | ||
| + | Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga" | ||
| + | Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor" | ||
| + | Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student" | ||
| + | Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair" | ||
| + | Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil" | ||
| + | Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress" | ||
| + | Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)" | ||
| + | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
| + | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
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| + | index = Math.floor(Math.random() * Q.length); | ||
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| + | document.writeln(Q[index]); | ||
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| + | } | ||
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| + | makeQuote(); | ||
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| + | </script> | ||
| + | </div> | ||
| + | </head> | ||
| + | </html> | ||
Latest revision as of 00:15, 30 August 2011
| Home | Project | Methods | Results | Data | Team | Notebook | Human Practices | Safety | Profile | Sponsors | Wix |
Contents |
Notebook
Click on a date to see what we have done!
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Gel 1:: HLyA / RBS+HLyD sample 1 / RBS+HLyD sample 2.
24 August 2011
New analysis of HlyA and HlyD
Objective: Quantification of HLyA and RBS+HLyD (volume of ladder applied 6 uL; sample: 5 uL DNA +1 uL buffer )
- Ladder: Bio-Rad 100bp – 10.000bp
- Samples:
- HLyA / RBS+HLyD sample 1 / RBS+HLyD sample 2
- Results:
| Gene | Total size (gene+vector) | Linear vector size | Gene size | Result |
|---|---|---|---|---|
| HlyA | 3027 pb | 2800 pb | 227 pb | Ran out of the gel… |
| RBS+HlyD | 4296 pb | 2800 pb | 1496 pb | Ok, sample 2 better than 1 |
-All the genes were purified using Fermentas kit.
Quantification of HlyA
Ladder and HLyA
Objective: quantification of HLyA (again!)
- Samples:
- Ladder and HlyA
Result:
- Very low concentration! New digestion to do with all the remaining from the Miniprep…
