Team:UNIPV-Pavia/Freezer

From 2011.igem.org

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Revision as of 09:41, 26 August 2011

UNIPV TEAM 2011

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FREEZER

For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name	Description	Store	Length	Strain	Vector	Quality Control	Creation Date
BBa_K081022	Plambda-RBS30-luxR-T(B1006)-pLux	BioBricks 2011	969	E. coli TOP10	pSB1A2	Seq ok (MIT)	29/06/2011
BBa_C0060	(aiiA) autoinducer inactivation enzyme	BioBricks 2011	789+25^*	E. coli TOP10	pSB1A2	Seq ok (MIT)	29/06/2011
BBa_B0015	(TT) double terminator	2009	129	E. coli TOP10	pSB1AK3	Seq ok (MIT)	FREEZER 2009 Dist
BBa_R0040	(pTet) TetR repressible promoter	2009	54	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
BBa_C0061	(luxI) autoinducer synthetase for AHL	BioBricks 2011	618+25^*	E. coli TOP10	pSB1A2	Seq ok (MIT)	30/06/2011
BBa_I13501	(mRFP-TT) Screening plasmid intermediate	requested from the Registry	843 (25 included)^*	E. coli TOP10	pSB1AK3	Seq ok (MIT)	FREEZER Requested 2011
BBa_B0030	RBS30 (eff=0.6) (SCAR BEFORE: 8bp)	2009	15	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
BBa_B0031	RBS31 (eff=0.07) derivative of BBa_0030	2009	14	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
BBa_B0032	RBS32 (eff=0.3)	2009	13	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
BBa_B0034	RBS34 (eff=1)	2009	12	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
BBa_I13521	(pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP	2010	923(25 included)^*	E. coli TOP10	pSB1A3	Seq ok (MIT)	FREEZER 2010 Dist
BBa_C0261	(RBS34-luxI) AHL-making Enzyme, luxI (+RBS)	BioBricks 2011	661 (25 included)^*	E. coli TOP10	pSB1A2	Seq ok (MIT)	07/07/2011
BBa_I13507	(RBS34-mRFP-TT) Screening plasmid intermediate	2009	861 (25 included)^*	E. coli TOP10	pSB1A2	Seq ok (MIT)	FREEZER 2009 Dist
pSB4C5	Low copy BioBrick standard vector - chloramphenicol antibiotic resistance	2009	3221	E. coli TOP10	pSB4C5	Seq ok (MIT)	FREEZER 2009 Dist
pSB3K3	Low to medium copy BioBrick standard vector - kanamycin resistance	BioBricks 2011	2750	E. coli TOP10	pSB3K3	Seq ok (MIT)	30/06/2011

Biobrick name	Description	Ligation	Length	Strain	Vector	Quality Control	Creation Date
E1-2	aiiA-TT	C0060(E-S)+B0015(E-X)	926+25^*	E. coli TOP10	pSB1AK3	Length OK	07/07/2011
E2-2	RBS30-LuxI	C0061(X-P)+B0030(S-P)	639+25^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E3-1	RBS31-LuxI	C0061(X-P)+B0031(S-P)	638+25^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E4-2	RBS32-LuxI	C0061(X-P)+B0032(S-P)	637+25^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E5-2	RBS30-mRFP-TT	I13501(X-P)+B0030(S-P)	864(25 included)^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E6-1	RBS31-mRFP-TT	I13501(X-P)+B0031(S-P)	863(25 included)^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E7-2	RBS32-mRFP-TT	I13501(X-P)+B0032(S-P)	862(25 included)^*	E. coli TOP10	pSB1A2	Length OK	07/07/2011
E8-3	Plambda-RBS30-luxR-T(B1006)-pLux	K081022(E-P)+4C5(E-P)	969(25 included)^*	E. coli TOP10	pSB4C5	Length OK	07/07/2011
E9-2	RBS30-aiiA-TT	E1(X-P)+B0030(S-P)	947+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	13/07/2011
E10-1	RBS31-aiiA-TT	E1(X-P)+B0031(S-P)	946+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	13/07/2011
E11-1	RBS32-aiiA-TT	E1(X-P)+B0032(S-P)	945+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	14/07/2011
E12-1	RBS34-aiiA-TT	E1(X-P)+ B0034(S-P)	946+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	14/07/2011
E13-1	pTet-RBS30-LuxI	E2(X-P)+R0040(S-P)	701+25^*	E. coli MGZ1	pSB1A2	Seq ok (BMR Genomics)	13/07/2011
E14	pTet-RBS31-LuxI	E3(X-P)+R0040(S-P)	700+25^*	E. coli TOP10	pSB1A2		FAILED
E15	pTet-RBS32-LuxI	E4(X-P)+R0040(S-P)	699+25^*	E. coli TOP10	pSB1A2		FAILED
E16-1	pTet-RBS34-LuxI	C0261(X-P)+R0040(S-P)	698??+25 (già 723)^*	E. coli MGZ1	pSB1A2	Seq ok (BMR Genomics)	13/07/2011
E17-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT	E5(X-P)+E8(S-P)	1841(25 included)^*	E. coli TOP10	pSB4C5	Seq ok (BMR Genomics)	13/07/2011
E18-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT	E6(X-P)+E8(S-P)	1840(25 included)^*	E. coli TOP10	pSB4C5	Seq ok (BMR Genomics)	13/07/2011
E19-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT	E7(X-P)+E8(S-P)	1839(25 included)^*	E. coli TOP10	pSB4C5	Seq ok (BMR Genomics)	13/07/2011
E20-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT	I13507(X-P)+E8(S-P)	1838(25 included)^*	E. coli TOP10	pSB4C5	Seq ok (BMR Genomics)	13/07/2011
E17-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT	E5(X-P)+E8(S-P)	1839^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E18-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT	E6(X-P)+E8(S-P)	1840^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E19-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT	E7(X-P)+E8(S-P)	1839^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E20-2	Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT	I13507(X-P)+E8(S-P)	1838^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E21-1	pTet-RBS30-mRFP-TT	E5(X-P)+R0040(S-P)	926(25 included)^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	14/07/2011
E22-2	pTet-RBS31-mRFP-TT	E6(X-P)+R0040(S-P)	925(25 included)^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	13/07/2011
E23-1	pTet-RBS32-mRFP-TT	E7(X-P)+R0040(S-P)	924(25 included)^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	14/07/2011
E24-2	pTet-RBS30-aiiA-TT	E9(X-P)+R0040(S-P)	1009+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	21/07/2011
E25-1	pTet-RBS31-aiiA-TT	E10(X-P)+R0040(S-P)	1008+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	21/07/2011
E26-2	pTet-RBS32-aiiA-TT	E11(X-P)+R0040(S-P)	1007+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	21/07/2011
E27-2	pTet-RBS34-aiiA-TT	E12(X-P)+R0040(S-P)	1006+25^*	E. coli TOP10	pSB1A2	Seq ok (BMR Genomics)	21/07/2011
E28-1	pTet-RBS30-luxI in pSB4C5	E13(E-P)+pSB4C5(E-P)	701+25^*	E. coli MGZ1	pSB4C5	Bad Seq	21/07/2011
E29	pTet-RBS31-luxI in pSB4C5	E14(E-P)+pSB4C5(E-P)	700+25^*	E. coli MGZ1	pSB4C5		FAILED
E30	pTet-RBS32-luxI in pSB4C5	E15(E-P)+pSB4C5(E-P)	699+25^*	E. coli MGZ1	pSB4C5		FAILED
E31-1	pTet-RBS34-luxI in pSB4C5	E16(E-P)+pSB4C5(E-P)	698+25^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	21/07/2011
E32-2	pTet-RBS30-mRFP-TT in pSB4C5	E21(E-P)+pSB4C5(E-P)	926(25 included)^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	21/07/2011
E33-2	pTet-RBS31-mRFP-TT in pSB4C5	E22(E-P)+pSB4C5(E-P)	925(25 included)^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	21/07/2011
E34-1	pTet-RBS32-mRFP-TT in pSB4C5	E23(E-P)+pSB4C5(E-P)	924(25 included)^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	21/07/2011
E35-2	pTet-RBS34-mRFP-TT in pSB4C5	I13521(E-P)+pSB4C5(E-P)	923(25 included)^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	21/07/2011
E36	pTet-J61002 in pSB4C5	pTet-J61002(E-P)+pSB4C5(E-P)	923^*	E. coli TOP10	pSB4C5	Red colonies	25/07/2011
E37-2	pTet-RBS30-aiiA-TT in pSB4C5	E24(E-P)+pSB4C5(E-P)	1009+25^*	E. coli MGZ1	pSB4C5		29/07/2011
E38-1	pTet-RBS31-aiiA-TT in pSB4C5	E25(E-P)+pSB4C5(E-P)	1008+25^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E39-1	pTet-RBS32-aiiA-TT in pSB4C5	E26(E-P)+pSB4C5(E-P)	1007+25^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E40-2	pTet-RBS34-aiiA-TT in pSB4C5	E27(E-P)+pSB4C5(E-P)	1006+25^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E41N-1	pTet-RBS31-luxI in pSB4C5	E36(S-P)+E3(X-P)	700+25^*	E. coli MGZ1	pSB4C5		04/08/2011
E42-1	pTet-RBS32-luxI in pSB4C5	E36(S-P)+ E4(X-P)	699+25^*	E. coli MGZ1	pSB4C5	Seq ok (BMR Genomics)	29/07/2011
E43-?	pTet-RBS30-luxI in pSB4C5	E36(S-P)+ E2(X-P)	701+25^*	E. coli MGZ1	pSB4C5	???	25/08/2011

^* The sequences of these parts contain a 25 bp barcode.

@@ Line 374: / Line 374: @@
        <td class="row">pSB1A2</td>
        <td class="row"></td>
-       <td class="row">13/07/2011</td>
+       <td class="row">FAILED</td>
     </tr>
@@ Line 385: / Line 385: @@
        <td class="row">pSB1A2</td>
        <td class="row"></td>
-       <td class="row">13/07/2011</td>
+       <td class="row">FAILED</td>
     </tr>
@@ Line 583: / Line 583: @@
        <td class="row">pSB4C5</td>
        <td class="row"></td>
-       <td class="row">???</td>
+       <td class="row">FAILED</td>
     </tr>
@@ Line 594: / Line 594: @@
        <td class="row">pSB4C5</td>
        <td class="row"></td>
-       <td class="row">???</td>
+       <td class="row">FAILED</td>
     </tr>