Team:UNIPV-Pavia/Freezer

From 2011.igem.org

(Difference between revisions)
Line 513: Line 513:
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
-
       <td class="row">1007<sup>*</sup></td>
+
       <td class="row">1009+25<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 524: Line 524:
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
-
       <td class="row">1008<sup>*</sup></td>
+
       <td class="row">1008+25<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 535: Line 535:
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
-
       <td class="row">1007<sup>*</sup></td>
+
       <td class="row">1007+25<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 546: Line 546:
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
-
       <td class="row">1006<sup>*</sup></td>
+
       <td class="row">1006+25<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 557: Line 557:
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">701<sup>*</sup></td>
+
       <td class="row">701+25<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 568: Line 568:
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">700<sup>*</sup></td>
+
       <td class="row">700+25<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 579: Line 579:
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">699<sup>*</sup></td>
+
       <td class="row">699+25<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 590: Line 590:
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">698<sup>*</sup></td>
+
       <td class="row">698+25<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>

Revision as of 15:05, 24 August 2011

UNIPV TEAM 2011

FREEZER


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969 E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789+25* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618+25* E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/2011
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843 (25 included)* E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) (SCAR BEFORE: 8bp) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) 2009 13 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923(25 included)* E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661 (25 included)* E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/2011
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861 (25 included)* E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/2011


Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 926+25* E. coli TOP10 pSB1AK3 Length OK 07/07/2011
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 639+25* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 638+25* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 637+25* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864(25 included)* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863(25 included)* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862(25 included)* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969(25 included)* E. coli TOP10 pSB4C5 Length OK 07/07/2011
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 947+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 946+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 945+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 701+25* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 700+25* E. coli TOP10 pSB1A2 13/07/2011
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 699+25* E. coli TOP10 pSB1A2 13/07/2011
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 698??+25 (già 723)* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1841(25 included)* E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840(25 included)* E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839(25 included)* E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838(25 included)* E. coli TOP10 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1839* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 926(25 included)* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925(25 included)* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924(25 included)* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1009+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1008+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1007+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1006+25* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 701+25* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 700+25* E. coli MGZ1 pSB4C5 ???
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 699+25* E. coli MGZ1 pSB4C5 ???
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 698+25* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923* E. coli TOP10 pSB4C5 Red colonies 25/07/2011
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1007* E. coli MGZ1 pSB4C5 29/07/2011
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1008* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1007* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1006* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E41N1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 700* E. coli MGZ1 pSB4C5 04/08/2011
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+ E4(X-P) 699* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E43-? pTet-RBS30-luxI in pSB4C5 E36(S-P)+ E2(X-P) 701* E. coli MGZ1 pSB4C5 ??? 25/08/2011

* The sequences of these parts contain a 25 bp barcode.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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