Team:UNICAMP-EMSE Brazil/Notebook/19 August 2011
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{{Template:UNICAMP-EMSE Brazil_Notebook}} | {{Template:UNICAMP-EMSE Brazil_Notebook}} | ||
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==19 August 2011== | ==19 August 2011== | ||
===Synthetic DNA sequences status=== | ===Synthetic DNA sequences status=== | ||
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*'''Agarose concentration:''' 0.8% | *'''Agarose concentration:''' 0.8% | ||
*'''Samples: ''' | *'''Samples: ''' | ||
- | :*GEL 1: | + | [[Image:UNICAMP-EMSE_Brazil_Gel1_19_08_11.png|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 1, 19/08/2011.''']] |
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+ | :*'''GEL 1:''' | ||
::*1. Const. Promoter1 (nd) | ::*1. Const. Promoter1 (nd) | ||
::*2. Const. Promoter1 | ::*2. Const. Promoter1 | ||
Line 25: | Line 26: | ||
::*15. RBS-IL12 2 | ::*15. RBS-IL12 2 | ||
::*16. Ladder | ::*16. Ladder | ||
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- | |||
[[Image:UNICAMP-EMSE_Brazil_Gel2_19_08_11.png|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 19/08/2011.''']] | [[Image:UNICAMP-EMSE_Brazil_Gel2_19_08_11.png|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 19/08/2011.''']] | ||
+ | |||
+ | :*'''GEL2:''' | ||
::*17. Ladder | ::*17. Ladder | ||
::*18. RBS-HylB 1 (nd) | ::*18. RBS-HylB 1 (nd) |
Revision as of 02:13, 24 August 2011
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Notebook
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19 August 2011
Synthetic DNA sequences status
Objective: Check the size of synthetic DNA sequences.
- Ladder: 100-10.000 bp (Fermentas)
- Agarose concentration: 0.8%
- Samples:
- GEL 1:
- 1. Const. Promoter1 (nd)
- 2. Const. Promoter1
- 3. flhDC1 (nd)
- 4. flhDC1
- 5. flhDC2
- 6. QseB-QseC1 (nd)
- 7. QseB-QseC1
- 8. RBS-SoxR1 (nd)
- 9. RBS-Sox1
- 10. RBS-IL10 1 (nd)
- 11. RBS-IL10 1
- 12. RBS-IL10 2
- 13. RBS-IL12 1 (nd)
- 14. RBS-IL12 1
- 15. RBS-IL12 2
- 16. Ladder
- GEL2:
- 17. Ladder
- 18. RBS-HylB 1 (nd)
- 19. RBS-HylB 2
- 20. HylA 1 (nd)
- 21. HylA 1
- 22. HylA 2
- 23. RBS-HylD 1 (nd)
- 24. RBS-HylD 1
- 25. RBS-HylD 2