Team:ETH Zurich/Biology/Journal

From 2011.igem.org

(Difference between revisions)

Revision as of 12:54, 22 August 2011

Can you feel the smoke tonight?

Week 1: 20.6-26.6

First meeting

Brainstorming

Week 2: 27.6-3.7

Brainstorming

Week 3: 4.7-10.7

Design of several Operons for AlcR and several PCR primer
Making of competent DH5-α cells

Week 4: 11.7-17.7

Cloning of the AlcR-testsystem

Week 5: 18.7-24.7

First test of the AlcR-system in 96-well plates

Week 6: 25.7-31.7

Transformation of the parts from the iGEM plates

Week 7: 1.8-7.8

Growth test of DH5-α with AlcR-system in flasks

Ligation and Transformation of
- λ_P and luxI
- λ_P and lacI
- P_lac and GFP_LVA
- P_lux and mCherry
- P_tet and CI -had to be redone because of point mutation in the primer (week 9)
- P_const and luxR

Week 8: 8.8-14.8

Synthesized LacI_M1 Gen arrived

Ligation and Transformation of
- P_lac-GFP_LVA and Terminator
- P_lux-mCherry and Terminator
- P_const-luxR and Terminator
- P_lac-GFP_LVA-Terminator and P_lux-mCherry-Terminator on one plasmid
- P_tet and LacI_M1
- P_tet-LacI_M1 and Terminator

Improving of the testsystem
- Exchange of same rare codons in AlcR by PCR
- His-tagging of AlcR
- GFP assam

Transformation of λ_P

Making of competent JM101 cells
- Check for transformation efficiency

Week 9: 15.8-21.8

Growth and repression test of AlcR-system with M9-medium
Ligation and Transformation of
- λ_P and lacI
- λ_P and luxI
- P_tet and CI
- λ_P-lacI and terminator
- λ_P-luxI and terminator

@@ Line 15: / Line 15: @@
 == ''' Week 3:   4.7-10.7''' ==
-* Design of several Operons for AlcR
+* Design of several Operons for AlcR and several PCR primer
+* Making of competent DH5-&alpha; cells
 == '''Week 4:   11.7-17.7''' ==
-*Cloning of the
+* Cloning of the AlcR-testsystem
-** AlcR-testsystem
 == '''Week 5:   18.7-24.7''' ==
 * First test of the AlcR-system in 96-well plates
-* Transformation of the parts from the iGEM plates
 == '''Week 6:   25.7-31.7''' ==
+* Transformation of the parts from the iGEM plates
 == '''Week 7:   1.8-7.8''' ==
@@ Line 39: / Line 39: @@
 ** P<sub>lac</sub> and GFP<sub>LVA</sub>
 ** P<sub>lux</sub> and mCherry
-** P<sub>tet</sub> and CI
+** P<sub>tet</sub> and CI -had to be redone because of point mutation in the primer (week 9)
 ** P<sub>const</sub> and luxR
@@ Line 45: / Line 45: @@
 == '''Week 8:   8.8-14.8''' ==
-Synthesized LacI<sub>M1</sub> Gen arrived
+* Synthesized LacI<sub>M1</sub> Gen arrived
 * Ligation and Transformation of
@@ Line 51: / Line 51: @@
 ** P<sub>lux</sub>-mCherry and Terminator
 ** P<sub>const</sub>-luxR and Terminator
+** P<sub>lac</sub>-GFP<sub>LVA</sub>-Terminator and P<sub>lux</sub>-mCherry-Terminator on one plasmid
+** P<sub>tet</sub> and LacI<sub>M1</sub>
+** P<sub>tet</sub>-LacI<sub>M1</sub> and Terminator
-* Exchange of same rare codons in AlcR by PCR
+* Improving of the testsystem
+** Exchange of same rare codons in AlcR by PCR
+** His-tagging of AlcR
+** GFP assam
 * Transformation of &lambda;<sub>P</sub>
+* Making of competent JM101 cells
+** Check for transformation efficiency
 == '''Week 9:   15.8-21.8''' ==
+* Growth and repression test of AlcR-system with M9-medium
-Third test of AlcR-system with M9-medium
+* Ligation and Transformation of
+** &lambda;<sub>P</sub> and lacI
+** &lambda;<sub>P</sub> and luxI
+** P<sub>tet</sub> and CI
+** &lambda;<sub>P</sub>-lacI and terminator
+** &lambda;<sub>P</sub>-luxI and terminator
 == '''Week 10:  22.8-28.8''' ==