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Team:ETH Zurich/Biology/Journal
From 2011.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
== '''Week 1: 20.6-26.6''' == | == '''Week 1: 20.6-26.6''' == | ||
| - | First meeting | + | * First meeting |
| - | Brainstorming | + | * Brainstorming |
== ''' Week 2: 27.6-3.7''' == | == ''' Week 2: 27.6-3.7''' == | ||
| + | |||
| + | * Brainstorming | ||
== ''' Week 3: 4.7-10.7''' == | == ''' Week 3: 4.7-10.7''' == | ||
| - | Design of several Operons for AlcR | + | * Design of several Operons for AlcR |
== '''Week 4: 11.7-17.7''' == | == '''Week 4: 11.7-17.7''' == | ||
| - | Cloning of the AlcR-testsystem | + | *Cloning of the |
| + | ** AlcR-testsystem | ||
== '''Week 5: 18.7-24.7''' == | == '''Week 5: 18.7-24.7''' == | ||
| - | First test of the AlcR-system in 96-well plates | + | * First test of the AlcR-system in 96-well plates |
| + | * Transformation of the parts from the iGEM plates | ||
== '''Week 6: 25.7-31.7''' == | == '''Week 6: 25.7-31.7''' == | ||
| Line 28: | Line 32: | ||
== '''Week 7: 1.8-7.8''' == | == '''Week 7: 1.8-7.8''' == | ||
| - | + | * Growth test of DH5-α with AlcR-system in flasks | |
| + | |||
| + | * Ligation and Transformation of | ||
| + | ** λ<sub>P</sub> and luxI | ||
| + | ** λ<sub>P</sub> and lacI | ||
| + | ** P<sub>lac</sub> and GFP<sub>LVA</sub> | ||
| + | ** P<sub>lux</sub> and mCherry | ||
| + | ** P<sub>tet</sub> and CI | ||
| + | ** P<sub>const</sub> and luxR | ||
| + | |||
== '''Week 8: 8.8-14.8''' == | == '''Week 8: 8.8-14.8''' == | ||
| - | Synthesized | + | Synthesized LacI<sub>M1</sub> Gen arrived |
| + | |||
| + | * Ligation and Transformation of | ||
| + | ** P<sub>lac</sub>-GFP<sub>LVA</sub> and Terminator | ||
| + | ** P<sub>lux</sub>-mCherry and Terminator | ||
| + | ** P<sub>const</sub>-luxR and Terminator | ||
| + | |||
| + | * Exchange of same rare codons in AlcR by PCR | ||
| + | |||
| + | * Transformation of λ<sub>P</sub> | ||
== '''Week 9: 15.8-21.8''' == | == '''Week 9: 15.8-21.8''' == | ||
| + | |||
Third test of AlcR-system with M9-medium | Third test of AlcR-system with M9-medium | ||
== '''Week 10: 22.8-28.8''' == | == '''Week 10: 22.8-28.8''' == | ||
Revision as of 12:15, 22 August 2011
Week 1: 20.6-26.6
- First meeting
- Brainstorming
Week 2: 27.6-3.7
- Brainstorming
Week 3: 4.7-10.7
- Design of several Operons for AlcR
Week 4: 11.7-17.7
- Cloning of the
- AlcR-testsystem
Week 5: 18.7-24.7
- First test of the AlcR-system in 96-well plates
- Transformation of the parts from the iGEM plates
Week 6: 25.7-31.7
Week 7: 1.8-7.8
- Growth test of DH5-α with AlcR-system in flasks
- Ligation and Transformation of
- λP and luxI
- λP and lacI
- Plac and GFPLVA
- Plux and mCherry
- Ptet and CI
- Pconst and luxR
Week 8: 8.8-14.8
Synthesized LacIM1 Gen arrived
- Ligation and Transformation of
- Plac-GFPLVA and Terminator
- Plux-mCherry and Terminator
- Pconst-luxR and Terminator
- Exchange of same rare codons in AlcR by PCR
- Transformation of λP
Week 9: 15.8-21.8
Third test of AlcR-system with M9-medium
