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AUGUST: WEEK 1
August, 1stFor each plate from the previous day, two colonies were picked. Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes. August, 2nd
Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:
J101-31, J101-E5, J101-E7 were transformed in MGZ1 competent cells. Digestions of E28AGAIN-1, E28AGAIN-2, E41AGAIN-1 and E42AGAIN-2 were performed for screening; a 5x mix was prepared and for each sample the volumes specified in the table below were used:
TOP10 competent cells, carrying BBa_T9002 construct, were transformed with ENTERO4C5 to confer resistance to Cloramphenicol (12.5 mg/ml).
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Team:UNIPV-Pavia/Calendar/August/week1
From 2011.igem.org
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Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes. | Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes. | ||
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| - | + | J101-31, J101-E5, J101-E7 were transformed in MGZ1 competent cells. | |
</p> | </p> | ||
<p> | <p> | ||
| - | Digestions of | + | Digestions of E28AGAIN-1, E28AGAIN-2, E41AGAIN-1 and E42AGAIN-2 were performed for screening; a 5x mix was prepared and for each sample the volumes specified in the table below were used: |
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| - | + | TOP10 competent cells, carrying <a href="http://partsregistry.org/wiki/index.php/Part:BBa_T9002">BBa_T9002</a> construct, were transformed with ENTERO4C5 to confer resistance to Cloramphenicol (12.5 mg/ml). | |
</p> | </p> | ||
Revision as of 11:15, 16 August 2011
