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Team:Lyon-INSA-ENS/Realisation/Week7Fr
From 2011.igem.org
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Revision as of 10:47, 5 August 2011
Semaine 7
From Monday the 25th of July to Friday the 29th of July 2011
Monday
Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.
Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).
Tuesday
Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.
Wednesday
PCR did not work.
Jeudi
Vendredi
La PCR a finalement marché avec la PHU 9104.
