Team:UNIPV-Pavia/Calendar/August/week1

From 2011.igem.org

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MODIFICARE: piccate J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate)
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Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:
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<table border="1">
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    <tr>
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      <td><b>Plasmid</b></td>
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      <td><b>DNA (ng/&mu;l)</b></td>
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      <td>E28AGAIN-1</td>
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      <td>21.4</td>
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      <td>E28AGAIN-2</td>
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      <td>21.0</td>
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      <td>E41AGAIN-1</td>
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      <td>33.7</td>
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      <td>E41AGAIN-2</td>
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      <td>27.7</td>
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      <td>E37-2</td>
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      <td>18.5</td>
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      <td>E38-1</td>
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      <td>26.0</td>
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      <td>E39-1</td>
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      <td>21.6</td>
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      <td>E40-2</td>
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      <td>19.9</td>
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  </tr>
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  <tr>
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      <td>E42-1</td>
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      <td>25.1</td>
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  </tr>
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</table>
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Revision as of 09:43, 3 August 2011

UNIPV TEAM 2011

March
M T W T F S S
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April
M T W T F S S
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18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
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2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
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13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
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4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
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3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

AUGUST: WEEK 1

August, 1st

For each plate from the previous day, two colonies were picked.

Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes.

Screening (E-S) bande 1-2-3.
Trasformati in MGZ1 da MiniPrep.

August, 2nd

MODIFICARE: piccate J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate)

Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:

Plasmid DNA (ng/μl)
E28AGAIN-1 21.4
E28AGAIN-2 21.0
E41AGAIN-1 33.7
E41AGAIN-2 27.7
E37-2 18.5
E38-1 26.0
E39-1 21.6
E40-2 19.9
E42-1 25.1
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Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia

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