Team:UNIPV-Pavia/Calendar/August/week1

From 2011.igem.org

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Next, screening digestion of purified plasmids was performed:
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Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 &mu;l test-tubes.
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Screening (E-S) bande 1-2-3.<br> Trasformati in MGZ1 da MiniPrep.
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      <td><b>Plasmid</b></td>
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      <td><b>Kind</b></td>
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      <td><b>DNA (&mu;l)</b></td>
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      <td><b>H<small><sub>2</sub></small>O (&mu;l)</b></td>
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      <td><b>Enzyme 1 (&mu;l)</b></td>
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      <td><b>Enzyme 2 (&mu;l)</b></td>
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      <td><b>Buffer H (&mu;l)</b></td>
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      <td><b>Final Volume (&mu;l)</b></td>
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      <td>E41-2</td>
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      <td>Insert</td>
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<a name="August.2C_2st"></a><h2> <span class="mw-headline">August, 2st</span></h2>
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      <td>9</td>
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      <td>12.5</td>
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      <td>0.5 EcoRI</td>
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      <td>0.5 Pstl</td>
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      <td>2.5</td>
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      <td>25</td>
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      <td>E42-1</td>
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      <td>Insert</td>
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      <td>9</td>
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      <td>12.5</td>
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      <td>0.5 EcoRI</td>
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      <td>0.5 Pstl</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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  <tr>
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      <td>E42-2</td>
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      <td>Insert</td>
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      <td>9</td>
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      <td>12.5</td>
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      <td>0.5 EcoRI</td>
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      <td>0.5 Pstl</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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Revision as of 09:31, 3 August 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

AUGUST: WEEK 1

August, 1st

For each plate from the previous day, two colonies were picked.

Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes.

Screening (E-S) bande 1-2-3.
Trasformati in MGZ1 da MiniPrep.

August, 2st

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Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia

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