Team:UNIPV-Pavia/Calendar/August/week1

(Difference between revisions)

Revision as of 09:16, 3 August 2011

UNIPV TEAM 2011

For each plate from the previous day, two colonies were picked.

Next, screening digestion of purified plasmids was performed:

@@ Line 13: / Line 13: @@
 <a name="August.2C_1st"></a><h2> <span class="mw-headline">August, 1st</span></h2>
+<p>
+For each plate from the previous day, two colonies were picked.
+</p>
+<p>
+Next, screening digestion of purified plasmids was performed:
+</p>
+<center>
+<table border="1">
+    <tr>
+       <td><b>Plasmid</b></td>
+       <td><b>Kind</b></td>
+       <td><b>DNA (&mu;l)</b></td>
+       <td><b>H<small><sub>2</sub></small>O (&mu;l)</b></td>
+       <td><b>Enzyme 1 (&mu;l)</b></td>
+       <td><b>Enzyme 2 (&mu;l)</b></td>
+       <td><b>Buffer H (&mu;l)</b></td>
+       <td><b>Final Volume (&mu;l)</b></td>
+   </tr>
+   <tr>
+      <td>E41-2</td>
+      <td>Insert</td>
+      <td>9</td>
+      <td>12.5</td>
+      <td>0.5 EcoRI</td>
+      <td>0.5 Pstl</td>
+      <td>2.5</td>
+      <td>25</td>
+   </tr>
+   <tr>
+      <td>E42-1</td>
+      <td>Insert</td>
+      <td>9</td>
+      <td>12.5</td>
+      <td>0.5 EcoRI</td>
+      <td>0.5 Pstl</td>
+      <td>2.5</td>
+      <td>25</td>
+   </tr>
+   <tr>
+      <td>E42-2</td>
+      <td>Insert</td>
+      <td>9</td>
+      <td>12.5</td>
+      <td>0.5 EcoRI</td>
+      <td>0.5 Pstl</td>
+      <td>2.5</td>
+      <td>25</td>
+   </tr>
+</table>
+</center>
 <table border="0" width="100%" class="menu">