Team:UNIPV-Pavia/Calendar/July/settimana5

From 2011.igem.org

(Difference between revisions)
Line 13: Line 13:
<a name="July.2C_25th"></a><h2> <span class="mw-headline">July, 25th</span></h2>
<a name="July.2C_25th"></a><h2> <span class="mw-headline">July, 25th</span></h2>
<p>
<p>
 +
 +
<p>
 +
Red colonies were observed from E36 plates, suggesting that the transformation was successful. A colony was picked form the plate.
 +
</p>
 +
<p>
<p>
Line 32: Line 37:
   <tr>
   <tr>
-
       <td>E9-2</td>
+
       <td>E24-2</td>
       <td>Insert</td>
       <td>Insert</td>
-
       <td>11.5</td>
+
       <td>13</td>
-
       <td>9</td>
+
       <td>7.5</td>
       <td>1 Xbal</td>
       <td>1 Xbal</td>
       <td>1 Pstl</td>
       <td>1 Pstl</td>
Line 44: Line 49:
   <tr>
   <tr>
-
       <td>E10-1</td>
+
       <td>E25-1</td>
       <td>Insert</td>
       <td>Insert</td>
-
       <td>10</td>
+
       <td>13.5</td>
-
       <td>10.5</td>
+
       <td>7</td>
       <td>1 Xbal</td>
       <td>1 Xbal</td>
       <td>1 Pstl</td>
       <td>1 Pstl</td>
Line 56: Line 61:
   <tr>
   <tr>
-
       <td>E11-1</td>
+
       <td>E26-2</td>
       <td>Insert</td>
       <td>Insert</td>
-
       <td>4</td>
+
       <td>14.5</td>
-
       <td>16.5</td>
+
       <td>6</td>
       <td>1 Xbal</td>
       <td>1 Xbal</td>
       <td>1 Pstl</td>
       <td>1 Pstl</td>
Line 68: Line 73:
   <tr>
   <tr>
-
       <td>E12-1</td>
+
       <td>E27-1</td>
       <td>Insert</td>
       <td>Insert</td>
-
       <td>10</td>
+
       <td>12.5</td>
-
       <td>10.5</td>
+
       <td>8</td>
       <td>1 Xbal</td>
       <td>1 Xbal</td>
       <td>1 PstI</td>
       <td>1 PstI</td>
Line 78: Line 83:
   </tr>
   </tr>
-
 
-
  <tr>
 
-
      <td>E13-1</td>
 
-
      <td>Insert</td>
 
-
      <td>6</td>
 
-
      <td>14.5</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Pstl</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
-
 
-
 
-
  <tr>
 
-
      <td>E16-1</td>
 
-
      <td>Insert</td>
 
-
      <td>4</td>
 
-
      <td>16.5</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Pstl</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
-
 
-
 
-
  <tr>
 
-
      <td>E21-1</td>
 
-
      <td>Insert</td>
 
-
      <td>7.5</td>
 
-
      <td>13</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Xbal</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
-
 
-
 
-
  <tr>
 
-
      <td>E22-2</td>
 
-
      <td>Insert</td>
 
-
      <td>10</td>
 
-
      <td>10.5</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Pstl</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td><a href="http://partsregistry.org/wiki/index.php/Part:BBa_I13521">BBa_I13521</a></td>
 
-
      <td>Insert</td>
 
-
      <td>12.5</td>
 
-
      <td>8</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Pstl</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td><a href="http://partsregistry.org/wiki/index.php/Part:BBa_R0040">BBa_R0040</a>-<a href="http://partsregistry.org/wiki/index.php/Part:BBa_J61002">BBa_J61002</a></td>
 
-
      <td>Vector</td>
 
-
      <td>20.5</td>
 
-
      <td>0</td>
 
-
      <td>1 EcoRl</td>
 
-
      <td>1 Pstl</td>
 
-
      <td>2.5</td>
 
-
      <td>25</td>
 
-
  </tr>
 
</table>
</table>

Revision as of 19:39, 28 July 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

JULY: WEEK 5

July, 25th

Red colonies were observed from E36 plates, suggesting that the transformation was successful. A colony was picked form the plate.

Digestions of previously purified plasmids were performed for ligations:

Plasmid Kind DNA (μl) H2O (μl) Enzyme 1 (μl) Enzyme 2 (μl) Buffer H (μl) Final Volume (μl)
E24-2 Insert 13 7.5 1 Xbal 1 Pstl 2.5 25
E25-1 Insert 13.5 7 1 Xbal 1 Pstl 2.5 25
E26-2 Insert 14.5 6 1 Xbal 1 Pstl 2.5 25
E27-1 Insert 12.5 8 1 Xbal 1 PstI 2.5 25
Previous week Next week

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia

Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5"