Team:UNIPV-Pavia/Calendar/July/settimana2

From 2011.igem.org

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<p>
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Digestion of previously purified plasmids were performed for ligations:
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</p>
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<center>
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<table border="1">
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    <tr>
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      <td><b>Plasmid</b></td>
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      <td>Kind</td>
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      <td><b>Purified Dna (&mul)</b></td>
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      <td><b>H<small><sub>2</sub></small>O (&mul)</b></td>
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      <td><b>Enzyme 1 (&mul)</b></td>
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      <td><b>Enzyme 2 (&mul)</b></td>
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      <td><b>Buffer H (&mul)</b></td>
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      <td><b>Final Volume(&mul)</b></td>
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  </tr>
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  <tr>
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      <td></html><partinfo>BBa_C0060</partinfo><html></td>
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      <td>Insert</td>
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      <td>16.5</td>
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      <td>4</td>
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      <td>1 EcoRI</td>
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      <td>1 SpeI</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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  <tr>
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      <td></html><partinfo>BBa_C0061</partinfo><html></td>
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      <td>Insert</td>
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      <td>13.2</td>
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      <td>7.3</td>
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      <td>1 XbaI</td>
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      <td>1 PstI</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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  <tr>
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      <td></html><partinfo>BBa_K081022</partinfo><html></td>
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      <td>Insert</td>
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      <td>15.7</td>
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      <td>4.8</td>
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      <td>1 EcoRI</td>
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      <td>1 PstI</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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 +
  <tr>
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      <td></html><partinfo>BBa_B0030</partinfo><html></td>
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      <td>Vector</td>
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      <td>13.2</td>
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      <td>7.3</td>
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      <td>1 SpeI</td>
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      <td>1 PstI</td>
 +
      <td>2.5</td>
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      <td>25</td>
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  </tr>
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 +
 +
  <tr>
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      <td></html><partinfo>BBa_B0031</partinfo><html></td>
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      <td>Vector</td>
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      <td>12.4</td>
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      <td>8.1</td>
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      <td>1 SpeI</td>
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      <td>1 PstI</td>
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      <td>2.5</td>
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      <td>25</td>
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  </tr>
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 +
 +
  <tr>
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      <td></html><partinfo>BBa_B0032</partinfo><html></td>
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      <td>Vector</td>
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      <td>9.5</td>
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      <td>11</td>
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      <td>1 SpeI</td>
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      <td>1 PstI</td>
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      <td>2.5</td>
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      <td>25</td>
 +
  </tr>
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 +
 +
  <tr>
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      <td></html><partinfo>BBa_B0015</partinfo><html></td>
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      <td>Vector</td>
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      <td>7.9</td>
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      <td>12.6</td>
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      <td>1 EcoRI</td>
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      <td>1 XbaI</td>
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      <td>2.5</td>
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      <td>25</td>
 +
  </tr>
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</table>
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 +
 +
  <tr>
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      <td></html><partinfo>pSB4C5</partinfo><html></td>
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      <td>Vector</td>
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      <td>3</td>
 +
      <td>17.5</td>
 +
      <td>1 EcoRI</td>
 +
      <td>1 PstI</td>
 +
      <td>2.5</td>
 +
      <td>25</td>
 +
  </tr>
 +
 +
</center>

Revision as of 14:51, 16 July 2011

UNIPV TEAM 2011

March
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June
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July
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August
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September
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JULY: WEEK 2

July, 4th

Digestion of previously purified plasmids were performed for ligations:

Plasmid Kind Purified Dna (&mul) H2O (&mul) Enzyme 1 (&mul) Enzyme 2 (&mul) Buffer H (&mul) Final Volume(&mul)
<partinfo>BBa_C0060</partinfo> Insert 16.5 4 1 EcoRI 1 SpeI 2.5 25
<partinfo>BBa_C0061</partinfo> Insert 13.2 7.3 1 XbaI 1 PstI 2.5 25
<partinfo>BBa_K081022</partinfo> Insert 15.7 4.8 1 EcoRI 1 PstI 2.5 25
<partinfo>BBa_B0030</partinfo> Vector 13.2 7.3 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0031</partinfo> Vector 12.4 8.1 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0032</partinfo> Vector 9.5 11 1 SpeI 1 PstI 2.5 25
<partinfo>BBa_B0015</partinfo> Vector 7.9 12.6 1 EcoRI 1 XbaI 2.5 25
<partinfo>pSB4C5</partinfo> Vector 3 17.5 1 EcoRI 1 PstI 2.5 25
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