"
Team:Baltimore/Notebook
From 2011.igem.org
(→Calendar) |
(→July) |
||
| Line 61: | Line 61: | ||
'''Week4:''' [[Tuesday, July 26]]; [[Wednesday, July 27]]; [[Thursday, July 28]]; [[Friday, July 29]]; [[Saturday, July 30]]; [[Sunday, July 31]]; | '''Week4:''' [[Tuesday, July 26]]; [[Wednesday, July 27]]; [[Thursday, July 28]]; [[Friday, July 29]]; [[Saturday, July 30]]; [[Sunday, July 31]]; | ||
| + | |||
| + | ====August==== | ||
| + | '''Week 1:''' [[Tuesday, August 2]]; [[Wednesday, August 3]]; [[Thursday, August 4]]; [[Friday, August 5]]; [[Saturday, August 6]]; [[Sunday, August 7]]; | ||
| + | |||
| + | '''Week 2:''' [[Tuesday, August 9]]; [[Wednesday, August 10]]; [[Thursday, August 11]]; [[Friday, August 12]]; [[Saturday, August 13]]; [[Sunday, August 14]]; | ||
| + | |||
| + | '''Week 3:''' [[Tuesday, August 16]]; [[Wednesday, August 17]]; [[Thursday, August 18]]; [[Friday, August 19]]; [[Saturday, August 20]]; [[Sunday, August 21]]; | ||
| + | |||
| + | '''Week 4:''' [[Tuesday, August 23]]; [[Wednesday, August 24]]; [[Thursday, August 25]]; [[Friday, August 26]]; [[Saturday, August 27]; [[Sunday, August 28]]; | ||
Revision as of 17:46, 14 July 2011
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Notebook
Run through for abstract and scheduling:
Start with plasmid with taq gene and pst1 gene
Remove pst1 site from taq coding sequence
- In order to remove the restriction site, do site directed mutagenesis (1day)
- PCR
- Digestion
- Transformation
- Screening (1day)
- Dilute DNA
- Colony PCR individually
- Digestion of PCR product with pst1
- Run gel ~2500bp
Add biobrick prefix/suffix to taq coding sequence
- PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
- Run gel
- Cut out of gel
- Cut with restriction enzyme
- Clean up DNA
- Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
- Transformation
Add a promoter, transcriptional terminator, ribosome binding site (RBS)
- Screen colonies (1 day)
- Colony PCR
- Restriction Digestion
- Clean up DNA
- Sequence (1 day)
Make taq protein
Compare it to other enzymes and make sure it works
Calendar
July
Week 2: Tuesday, July 12; Wednesday, July 13; Thursday, July 14; Friday, July 15; Saturday, July 16; Sunday, July 17;
Week 3: Tuesday, July 19; Wednesday, July 20; Thursday, July 21; Friday, July 22; Saturday, July 23; Sunday, July 24;
Week4: Tuesday, July 26; Wednesday, July 27; Thursday, July 28; Friday, July 29; Saturday, July 30; Sunday, July 31;
August
Week 1: Tuesday, August 2; Wednesday, August 3; Thursday, August 4; Friday, August 5; Saturday, August 6; Sunday, August 7;
Week 2: Tuesday, August 9; Wednesday, August 10; Thursday, August 11; Friday, August 12; Saturday, August 13; Sunday, August 14;
Week 3: Tuesday, August 16; Wednesday, August 17; Thursday, August 18; Friday, August 19; Saturday, August 20; Sunday, August 21;
Week 4: Tuesday, August 23; Wednesday, August 24; Thursday, August 25; Friday, August 26; [[Saturday, August 27]; Sunday, August 28;
