"
Team:EPF-Lausanne/Protocols
From 2011.igem.org
(Difference between revisions)
(→Molecular Biology) |
|||
| Line 9: | Line 9: | ||
* [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | ||
* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | ||
| + | * [[Team:EPF-Lausanne/Protocols/Primers preparation|Primers preparation]]. | ||
== Microfluidics == | == Microfluidics == | ||
Revision as of 14:58, 8 July 2011
Protocols
Molecular Biology
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Linear template- TetR.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
- Competent Cells.
- Competent Cells. Protocol II (Inoue method).
- Transformation.
- Primers preparation.
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
