Revision as of 17:29, 28 October 2011

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Wiki Highlights

Read our project description and summary on our Project page: more details on how team members completed work for the three sections of our project are on Design, Synthesize, and Test pages, respectively.

Results of all parts of our project (including our biobricks) are located on our Results page, along with an Acknowledgments section.

Human practices can be found on our Human Practices page.

Please be sure to stop by our poster during the poster session with any questions.

Accomplishments

Experimental Results:

✓ Researched zinc finger proteins and choose 6 novel, clinically relevant target sequences
✓ Generated 55,000 zinc finger protein sequences using bioinformatics
✓ Used three recently developed technologies together for the first time: a foundational advance
✓ Expressed those 55,000 sequences in E.coli, with a sub-library for each target
✓ Created a genomic one-hybrid selection system sensitive enough to detect one in a million hits
✓ Found up to 15 novel zinc finger proteins

Biobricks and Protocols:

✓ Submitted 5 Biobricks to the registry, including our one-hybrid selection strain
✓ Created several chassis for our Biobricks, including our one-hybrid selection strain
✓ Used and shared our easy-to-follow protocols
✓ Made protocols, Biobricks, and source code freely available, so that others can adapt them for other
projects

Human Practices

✓ Interviewed zinc finger researchers Dr. Keith Joung and Dr. George Church
✓ Researched intellectual property and how it applies to zinc finger proteins
✓ Created a timeline and case study of zinc finger intellectual property
✓ Handed out IP pamphlets and chassis data sheets at our poster presentation

@@ Line 23: / Line 23: @@
 ==Experimental Results:==
-'''✓''' Researched zinc finger proteins and choose 6 novel, <html><x>clinically relevant target sequences </x></html><br><br>
+'''✓''' Researched zinc finger proteins and choose 6 novel, <html><x>clinically relevant target sequences </x></html><br>
-'''✓''' Generated <html><x>55,000</x></html> zinc finger protein sequences using <html><x>bioinformatics</x></html> <br><br>
+'''✓''' Generated <html><x>55,000</x></html> zinc finger protein sequences using <html><x>bioinformatics</x></html> <br>
-'''✓''' Used three recently developed technologies together for the first time: a <html><x>foundational advance</x></html><br><br>
+'''✓''' Used three recently developed technologies together for the first time: a <html><x>foundational advance</x></html><br>
-'''✓''' <html><x>Expressed</x></html> those 55,000 sequences <html><x>in E.coli</x></html>, with a sub-library for each target <br><br>
+'''✓''' <html><x>Expressed</x></html> those 55,000 sequences <html><x>in E.coli</x></html>, with a sub-library for each target <br>
-'''✓''' Created a <html><x>genomic one-hybrid selection system</x></html> sensitive enough to detect <html><x>one in a million hits</x></html> <br><br>
+'''✓''' Created a <html><x>genomic one-hybrid selection system</x></html> sensitive enough to detect <html><x>one in a million hits</x></html> <br>
-'''✓''' Found up to <html><x>15 novel zinc finger proteins</x></html> <br><br>
+'''✓''' Found up to <html><x>15 novel zinc finger proteins</x></html> <br>
 ==Biobricks and Protocols:==
 '''✓''' Submitted <html><x>5 Biobricks</x></html> to the registry, including our one-hybrid selection strain<br>
 '''✓''' Created several <html><x>chassis</x></html> for our Biobricks, including our one-hybrid selection strain<br>
-'''✓''' Used and shared our <html><x>easy-to-follow <html><x>protocols<br>
+'''✓''' Used and shared our <html><x>easy-to-follow protocols</x></html><br>
 '''✓''' Made protocols, Biobricks, and source code <html><x>freely available</x></html>, so that others can adapt them for other <br>projects <br>

Team:Harvard/Judging

From 2011.igem.org