Wiki Highlights

• Read our project description and summary on our Project page: more details on how team members completed work for the three sections of our project are on Design, Synthesize, and Test pages, respectively.

• Results of all parts of our project (including our biobricks) are located on our Results page, along with an Acknowledgments section.

• Human practices can be found on our Human Practices page.

• Please be sure to stop by our poster during the poster session with any questions.

Accomplishments

✓ Researched zinc finger proteins and choose 6 novel, clinically relevant target sequences

✓ Generated 55,000 zinc finger protein sequences using bioinformatics

✓ Expressed those 55,000 sequences in E.coli, with a sub-library for each target

✓ Created a genomic one-hybrid selection system sensitive enough to detect one in a million hits

✓ Found up to 15 novel zinc finger proteins

✓ Researched intellectual property and how it applies to zinc finger proteins

✓ Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects