Team:Harvard/Judging
From 2011.igem.org
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=Accomplishments= | =Accomplishments= | ||
- | '''✓''' Researched zinc finger proteins and choose 6 novel, <div style=" | + | '''✓''' Researched zinc finger proteins and choose 6 novel, <div style="color:#C22227">clinically relevant target sequences </div><br> |
'''✓''' Generated 55,000 zinc finger protein sequences using bioinformatics <br> | '''✓''' Generated 55,000 zinc finger protein sequences using bioinformatics <br> | ||
'''✓''' Expressed those 55,000 sequences in E.coli, with a sub-library for each target <br> | '''✓''' Expressed those 55,000 sequences in E.coli, with a sub-library for each target <br> |
Revision as of 15:41, 28 October 2011
Wiki Highlights
- Read our project description and summary on our Project page: more details on how team members completed work for the three sections of our project are on Design, Synthesize, and Test pages, respectively.
- Results of all parts of our project (including our biobricks) are located on our Results page, along with an Acknowledgments section.
- Human practices can be found on our Human Practices page.
- Please be sure to stop by our poster during the poster session with any questions.
Accomplishments
✓ Researched zinc finger proteins and choose 6 novel,clinically relevant target sequences
✓ Generated 55,000 zinc finger protein sequences using bioinformatics
✓ Expressed those 55,000 sequences in E.coli, with a sub-library for each target
✓ Created a genomic one-hybrid selection system sensitive enough to detect one in a million hits
✓ Found up to 15 novel zinc finger proteins
✓ Researched intellectual property and how it applies to zinc finger proteins
✓ Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects