Team:ETH Zurich/Achievements/Data page
From 2011.igem.org
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- | + | = Data page = | |
- | + | '''SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.''' | |
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- | + | = How Our System Works = | |
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[[File:ETHZ Data page.png|850px|left|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]] | [[File:ETHZ Data page.png|850px|left|thumb|'''Figure 1: Moduls and molecular mechanism of SmoColi induced with different moleculs,''' depending on the input, either a spatially limited GFP or global RFP signal is produced in the channel. ]] | ||
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- | + | = Data For Our Favorite New Parts = | |
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#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | #[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | ||
#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy number test|Characterization]]) | #[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy number test|Characterization]]) | ||
#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor |Characterization]]) | #[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor |Characterization]]) | ||
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- | + | = We've also characterized the following parts = | |
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#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | #[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]]) | ||
#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor|Characterization]]) | #[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor|Characterization]]) | ||
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Revision as of 17:03, 25 October 2011
Data page
SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
Data For Our Favorite New Parts
- [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
- [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. (Characterization)
- [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter, BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization)
We've also characterized the following parts
- [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
- [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)