Team:Osaka/week13

From 2011.igem.org

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==October 25 (Tue)==
==October 25 (Tue)==
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# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
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# Electrophoresis of digests
 +
# To liquid culture:130,131,132,133,134,135,136,137,142
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# Colony counts (Tolerance assay)
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# Plating (Tolerance assay)
==October 26 (Wed)==
==October 26 (Wed)==

Revision as of 16:15, 25 October 2011


October 23 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. Gel extraction
  5. Ligation
    • 142(C):126(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:130,131,132,133,134,138,139,140,141
  7. Transformation of ligation products

October 24 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
    • 131,133,134 x
  4. Gel extraction
  5. Ligation
    • 143(C):132(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:131,133,134,142
  7. Plating (Tolerance assay)
  8. Transformation of ligation products

October 25 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. To liquid culture:130,131,132,133,134,135,136,137,142
  5. Colony counts (Tolerance assay)
  6. Plating (Tolerance assay)

October 26 (Wed)

October 27 (Thu)

October 28 (Fri)

October 29 (Sat)

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