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Team:Osaka/week13
From 2011.igem.org
(Difference between revisions)
| Line 19: | Line 19: | ||
# Gel extraction | # Gel extraction | ||
# Ligation | # Ligation | ||
| - | #* 143(C): | + | #* 143(C):132(upstream)+118(downstream)+1-3A(vector) |
# To liquid culture:131,133,134,142 | # To liquid culture:131,133,134,142 | ||
# Plating (Tolerance assay) | # Plating (Tolerance assay) | ||
Revision as of 15:22, 24 October 2011
October 23 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- Gel extraction
- Ligation
- 142(C):126(upstream)+118(downstream)+1-3A(vector)
- To liquid culture:130,131,132,133,134,138,139,140,141
- Transformation of ligation products
October 24 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Electrophoresis of digests
- 131,133,134 x
- Gel extraction
- Ligation
- 143(C):132(upstream)+118(downstream)+1-3A(vector)
- To liquid culture:131,133,134,142
- Plating (Tolerance assay)
- Transformation of ligation products
October 25 (Tue)
October 26 (Wed)
October 27 (Thu)
October 28 (Fri)
October 29 (Sat)
week 1|2|3|4|5|6|7|8|9|10|11|12|13
