Team:KAIT Japan/Project

From 2011.igem.org

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== '''Back Ground''' ==
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Bacteria detect the concentration of its friends, called Quorum Sensing, and the
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sensing system is one of the communication tools between microbes. It is related also
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to formation of biofilm and to pathogenicity. As the Quorum Sensing of Gram-negative
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<title>無題 2</title>
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bacterias, Acyl-Homoserine Lactone ( AHL ) is released to recognize each other. 2007,
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the team of Chiba University made the vacteria production of aiia to decrease the AHL.
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We considered application of the system to make a colony layer on a colony layer by
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depressing of Quorum Sensing.
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== '''Purpose '''　==
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We make bacteria that have transgene of AiiA, and get the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and General, colony and colony don’t approach by AHL.
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Our projects were done to approach and overlap.
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<body>
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=== '''Quorum Sensing''' ===
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<p class="style1">Project</p>
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Quorum sensing is a system of stimulus and response correlated
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<p class="style2"><strong>Back Ground</strong></p>
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to population density. Many species of bacteria use quorum
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<p class="style3"><strong>Bacteria detect the concentration of its friends,
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sensing to coordinate gene expression according to the density of
+
called Quorum Sensing, and the sensing system is one of the communication tools
-
their local population. In similar fashion, some social insects use
+
between microbes. It is related also to formation of biofilm and to
-
quorum sensing to determine where to nest.
+
pathogenicity. <br>As the Quorum Sensing of Gram-negative bacterias,
 +
Acyl-Homoserine Lactone <br>(AHL ) is released to recognize each other. 2007,
 +
the team of Chiba University made the vacteria production of aiia to decrease
 +
the AHL.<br>We considered application of the system to make a colony layer on a
 +
colony layer by<br>depressing of Quorum Sensing.</strong></p>
 +
<p class="style2"><strong>Purpose</strong></p>
 +
<p class="style3"><strong>We make bacteria that have transgene of AiiA, and get
 +
the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and
 +
General, colony and colony don’t approach by AHL.<br>Our projects were done to
 +
approach and overlap.</strong></p>
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<p class="style3">&nbsp;</p>
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<p class="style3">&nbsp;</p>
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<p class="style4"><strong>W</strong><span id="result_box" class="short_text" lang="en"><span class="hps"><strong>ord</strong></span></span></p>
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<p class="style5"><strong>Quorum Sensing</strong></p>
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<p class="style3"><strong>Quorum sensing is a system of stimulus and response  
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correlated to population density. <br>Many species of bacteria use quorum  
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sensing to coordinate gene expression according to the density of their local  
 +
population. In similar fashion, some social insects use quorum sensing to  
 +
determine where to nest.</strong></p>
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<p class="style5"><strong>AHL and AiiA</strong></p>
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<p class="style3"><strong>AHL is [N-] acyl-homoserine lactones. <br>AHL is a
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small transmitter that is used in quorum sensing system autoinducer (AI) is
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known as. AHL is present in many gram-negative bacteria<br>In low cell density,
 +
AHL becomes low concentration, and AHL becomes high concentration in high cell
 +
density. <br>At high cell densities, the AIs can accumulate to a concentration
 +
sufficient for activation of related regulatory genes. Because the concentration
 +
of AHL&#39;s is a key factor in determining virulence gene expression in several
 +
pathogenic bacteria, it is possible to develop a strategy for disease control by
 +
controlling production of AIs or eliminating AIs produced by pathogenic
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bacteria.<br><br>AiiA, an enzyme that inactivates the acylhomoserine lactone
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quorum-sensing signal.<br></strong></p>
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</body>
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</html>
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=== '''AHL　and AiiA''' ===
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AHL is [N-] acyl-homoserine lactones.
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hese are small signalling molecules which are employed in "quorum sensing" systems. They are also known as autoinducers (AIs) and are present in many Gram-negative bacteria.
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"Although the target genes regulated by AIs are extremely varied, the basic mechanism of AIs biosynthesis and gene regulation seems to be conserved in different bacteria. The general feature of gene regulation by AIs is cell-density dependence, also known as "quorum sensing".
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At low cell densities, the AIs are at low concentrations, and, at high cell densities, the AIs can accumulate to a concentration sufficient for activation of related regulatory genes. Because the concentration of AHL's is a key factor in determining virulence gene expression in several pathogenic bacteria, it is possible to develop a strategy for disease control by controlling production of AIs or eliminating AIs produced by pathogenic bacteria.
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AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal.
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Revision as of 01:51, 6 October 2011

無題 2

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無題 2

Project

Back Ground

Bacteria detect the concentration of its friends, called Quorum Sensing, and the sensing system is one of the communication tools between microbes. It is related also to formation of biofilm and to pathogenicity.
As the Quorum Sensing of Gram-negative bacterias, Acyl-Homoserine Lactone
(AHL ) is released to recognize each other. 2007, the team of Chiba University made the vacteria production of aiia to decrease the AHL.
We considered application of the system to make a colony layer on a colony layer by
depressing of Quorum Sensing.

Purpose

We make bacteria that have transgene of AiiA, and get the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and General, colony and colony don’t approach by AHL.
Our projects were done to approach and overlap.

 

 

Word

Quorum Sensing

Quorum sensing is a system of stimulus and response correlated to population density.
Many species of bacteria use quorum sensing to coordinate gene expression according to the density of their local population. In similar fashion, some social insects use quorum sensing to determine where to nest.

AHL and AiiA

AHL is [N-] acyl-homoserine lactones.
AHL is a small transmitter that is used in quorum sensing system autoinducer (AI) is known as. AHL is present in many gram-negative bacteria
In low cell density, AHL becomes low concentration, and AHL becomes high concentration in high cell density.
At high cell densities, the AIs can accumulate to a concentration sufficient for activation of related regulatory genes. Because the concentration of AHL's is a key factor in determining virulence gene expression in several pathogenic bacteria, it is possible to develop a strategy for disease control by controlling production of AIs or eliminating AIs produced by pathogenic bacteria.

AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal.

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