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We tried to overlap colony on colony. Generally, colony and colony don’t overlap and approach each other, because of the cell-cell communications. Bacterias also have a system, so called quorum sensing, to control of production of signal chemicals depend on the bacteria’s density.

We pay attention quorum sensing system. Bacterias produce and secret autoinducer against other bacterias by quorum sensing system, as a result of the system, bacteria senses other bacterias. Most of Gram-negative bacterias make use of autoinducer named AHL. We applied E. coli to our experiment and we use aiia which is catabolic enzyme to inhibit AHL. We tried to make a recombinant bacteria to cancelled sensing of other bacterias and expected to form the colony of bacterias on the other bacterias.