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Team:KAIT Japan/Project
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Project
Back Ground
Bacteria detect the concentration of its friends,
called Quorum Sensing, and the sensing system is one of the communication tools
between microbes. It is related also to formation of biofilm and to
pathogenicity.
As the Quorum Sensing of Gram-negative bacterias,
Acyl-Homoserine Lactone
(AHL ) is released to recognize each other. 2007,
the team of Chiba University made the vacteria production of aiia to decrease
the AHL.
We considered application of the system to make a colony layer on a
colony layer by
depressing of Quorum Sensing.
Purpose
We make bacteria that have transgene of AiiA, and get
the bacteria to produce abundance of AiiA.The abundance of AiiA inhibit AHL,and
General, colony and colony don’t approach by AHL.
Our projects were done to
approach and overlap.
Quorum Sensing Quorum sensing is a system of stimulus and response
correlated to population density. AHL and AiiA AHL is [N-] acyl-homoserine lactones. Autoinducer Autoinducers are chemical signaling molecules that are
produced and used by bacteria participating in quorum sensing. Word
Many species of bacteria use quorum
sensing to coordinate gene expression according to the density of their local
population. In similar fashion, some social insects use quorum sensing to
determine where to nest.
AHL is a
small transmitter that is used in quorum sensing system autoinducer (AI) is
known as. AHL is present in many gram-negative bacteria
In low cell density,
AHL becomes low concentration, and AHL becomes high concentration in high cell
density.
At high cell densities, the AIs can accumulate to a concentration
sufficient for activation of related regulatory genes. Because the concentration
of AHL's is a key factor in determining virulence gene expression in several
pathogenic bacteria, it is possible to develop a strategy for disease control by
controlling production of AIs or eliminating AIs produced by pathogenic
bacteria.
AiiA, an enzyme that inactivates the acylhomoserine lactone
quorum-sensing signal.
