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Team:Tokyo Metropolitan/Notebook/S02
From 2011.igem.org
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| + | Ligation HNS(wt) and BBa_I20260 | ||
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| + | Transformation to JM109 | ||
= -Visualization- = | = -Visualization- = | ||
Latest revision as of 16:19, 5 October 2011
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-Speeding up-
Ligation HNS(wt) and BBa_I20260
Transformation to JM109
-Visualization-
Ethanol precipitation of J23100,I714051.
Concentration of J23100,I714051 was still low.
@transformation OriTr+GFP and OriT, but failure
-Targeting-
-Plasmid extraction
Used QlaprepSpinMiniprepkit
Results
J23100
Jaiko 123
Jian 82
P0440
Pisuke 158
Pichiku 155
(ng/ul)
-Electrophoresis
Checked for K317037 and J23100
Results
K317037 and J23100 had cut
-Gel extraction
Gel extracted to DNA refined
Results for concentration
Kenedy 20
Keit 32
Jason 0
Jiro 10
-Digestion
J23100: S and P
P0440: X adn P
-Preculture
Precultured K317037, then incubated at 37 deg C.
Made 50*TAEBuffer
