-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.

Results measured concentration

No. 29 of J23100-K317037 was 150.

No. 19 of J23100-P0440 was 138.

J22005

I. 105

II. 155

III. 62

concentration was ng/ul

-Treat restriction enzyme

J23100-K317037 -> S and P

J23100-P0440 -> E and P

K283030 -> X and P

J22005 -> N

pSB1C3 -> E and P

incubated at 37 deg C after treat with the preceding enzymes.

-Setrilization for liquid medium

Setrilised for nine tubes 4 ml.

-Visualization-

-Plasmid extraction

Promoter

Suppressed expression system

RFP

-DNA refinig

Above samples

@plasmid extraction, DNA refine, DNA densitometry, restrictin emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP-OriTr

@excess preculture medium move to media(Amp).