-Plasmid extraction and DNA refining
Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.
Results measured concentration
No. 29 of J23100-K317037 was 150.
No. 19 of J23100-P0440 was 138.
concentration was ng/ul
-Treat restriction enzyme
J23100-K317037 -> S and P
J23100-P0440 -> E and P
K283030 -> X and P
J22005 -> N
pSB1C3 -> E and P
incubated at 37 deg C after treat with the preceding enzymes.
-Setrilization for liquid medium
Setrilised for nine tubes 4 ml.
Suppressed expression system
@plasmid extraction, DNA refine, DNA densitometry, restrictin emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP-OriTr
@excess preculture medium move to media(Amp).