Team:Tokyo Metropolitan/Notebook/S30

From 2011.igem.org

(Difference between revisions)
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Ligation might be failed. Results from sequence, some samples lost oriTr.
Ligation might be failed. Results from sequence, some samples lost oriTr.
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-Colony PCR
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Checked for results promoter - suppressed expression system (Cm)

Latest revision as of 13:50, 5 October 2011


-Visualization-

-oriTr-GFP+oriTr Electophoresis to check for colony PCR

-oriTr-GFP+oriTr Plasmid extraction to DNA refining

-oriTr-GFP+oriTr Digestion

-oriTr-GFP+oriTr Electrophoresis


Results

Ligation might be failed. Results from sequence, some samples lost oriTr.



-Colony PCR

Checked for results promoter - suppressed expression system (Cm)