Team:Tokyo Metropolitan/Notebook/S30
From 2011.igem.org
(Difference between revisions)
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Ligation might be failed. Results from sequence, some samples lost oriTr. | Ligation might be failed. Results from sequence, some samples lost oriTr. | ||
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+ | -Colony PCR | ||
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+ | Checked for results promoter - suppressed expression system (Cm) |
Latest revision as of 13:50, 5 October 2011
-Visualization-
-oriTr-GFP+oriTr Electophoresis to check for colony PCR
-oriTr-GFP+oriTr Plasmid extraction to DNA refining
-oriTr-GFP+oriTr Digestion
-oriTr-GFP+oriTr Electrophoresis
Results
Ligation might be failed. Results from sequence, some samples lost oriTr.
-Colony PCR
Checked for results promoter - suppressed expression system (Cm)