Team:Tokyo Metropolitan/Notebook/S13

From 2011.igem.org

(Difference between revisions)
(-Visualization-)
 
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@transformation OG+O ②
@transformation OG+O ②
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-Plasmid extraction
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Promoter and suppressed expression system
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-DNA refining
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Above samples
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-Digestion
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Above samples
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-Electrophoresis
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Above samples
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-Digestion
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Promoter: Spe and Pst
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Suppressed expression system: Xba and Pst
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Latest revision as of 13:29, 5 October 2011


-Visualization-

@confirmation of transformation (yesterday)

@restriction enzyme to pSB1C3(backbone for parts) (EcoRI, SpeⅠ)

@electrophore pSB1C3, O+G, total 4 samples.

@restriction enzyme to pSB1C3 (EcoRI, SpeⅠ)

@ligation OG+O ①

@transformation OG+O ①

@ligation OG+O ②

@transformation OG+O ②


-Plasmid extraction

Promoter and suppressed expression system


-DNA refining

Above samples


-Digestion

Above samples


-Electrophoresis

Above samples


-Digestion

Promoter: Spe and Pst

Suppressed expression system: Xba and Pst


-Targetting-

@electrophoresis J23100-K317037, K283030, J22005
J23100-K317037, K283030 find binds.
J22005 not find bind.

@gel extraction and confirmation binds by electrophoresis J23100-K317037, K283030

@ligation J23100-K317037 and K283030

@transformation J23100-K317037-K283030