Team:Tokyo Metropolitan/Notebook/S09

From 2011.igem.org

(Difference between revisions)
Line 48: Line 48:
Setrilised for nine tubes 4 ml.
Setrilised for nine tubes 4 ml.
 +
 +
 +
= -Visualization- =
 +
-Plasmid extraction
 +
 +
Promoter
 +
 +
Suppressed expression system
 +
 +
RFP
 +
 +
 +
-DNA refinig
 +
 +
Above samples

Revision as of 13:25, 5 October 2011

-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.


Results measured concentration

No. 29 of J23100-K317037 was 150.

No. 19 of J23100-P0440 was 138.


J22005

I. 105

II. 155

III. 62


concentration was ng/ul


-Treat restriction enzyme

J23100-K317037 -> S and P

J23100-P0440 -> E and P

K283030 -> X and P

J22005 -> N

pSB1C3 -> E and P

incubated at 37 deg C after treat with the preceding enzymes.


-Setrilization for liquid medium

Setrilised for nine tubes 4 ml.


-Visualization-

-Plasmid extraction

Promoter

Suppressed expression system

RFP


-DNA refinig

Above samples