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Team:Tokyo Metropolitan/Notebook/A16
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Revision as of 09:42, 5 October 2011
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-Visualization-
Maked liquid medium.
100ml
Yeast extract 0.5 g
Peptone 1 g
NaCl 1 g
Amp 100 µl
According to protocol.
Did not sterilize before autoclave, just in the bottle.
Add Amp and use for pre-culture.
Maked agarose gel.
Agarose 1.2 g
TAE Buffer 100 ml
1. Melted agarose and TAE Buffer in conical flask by microwave oven.
2. Poured into a mould pierced a comb.
3. After set kept in TAE Buffer.
Pre cultured colony of GFP + OriTr.
